Paul V, Jayakumar A R
Department of Pharmacology & Environmental Toxicology, Dr. A. L. M. Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai.
Indian J Physiol Pharmacol. 1999 Oct;43(4):479-85.
The effect of L-arginine (840 mg/kg) pre- (30 min before challenge) and post-treatment (5 min after challenge) period was tested on picrotoxin-induced increase in ammonia concentrations in brain regions (cerebral cortex, brain stem and cerebellum) and the accompanying convulsive responses in adult male rats. The combined effect of L-arginine and diazepam was also tested against picrotoxin-induced convulsions. Picrotoxin-induced increase in ammonia was reverted partially by L-arginine pretreatment. However, L-arginine pretreatment did not show anticonvulsant effect independently or concurrently with diazepam. On the other hand, L-arginine post-treatment reverted ammonia to control level in all brain regions. A partial but significant inhibition of convulsion responses was found in these animals. The combined effect of diazepam and L-arginine post-treatment was much greater than that produced by these agents independently. These findings suggest that ammonia has a partial but significant participation in the convulsant action of picrotoxin. L-arginine has a potential to revert brain ammonia to control level in picrotoxin-treated animals and thereby it has produced a partial protection. The data further indicate that the duration of action of L-arginine is considerably short and has an additive anticonvulsant action with diazepam.
在成年雄性大鼠中，测试了L-精氨酸（840毫克/千克）在预处理（激发前30分钟）和后处理（激发后5分钟）阶段对印防己毒素诱导的脑区（大脑皮层、脑干和小脑）氨浓度升高以及伴随的惊厥反应的影响。还测试了L-精氨酸和地西泮联合使用对印防己毒素诱导惊厥的效果。L-精氨酸预处理可部分逆转印防己毒素诱导的氨升高。然而，L-精氨酸预处理单独或与地西泮同时使用均未显示出抗惊厥作用。另一方面，L-精氨酸后处理可使所有脑区的氨恢复到对照水平。在这些动物中发现惊厥反应受到部分但显著的抑制。地西泮和L-精氨酸后处理的联合作用远大于这两种药物单独使用所产生的作用。这些发现表明，氨在印防己毒素的惊厥作用中起部分但显著的作用。L-精氨酸有潜力使印防己毒素处理动物的脑氨恢复到对照水平，从而产生部分保护作用。数据进一步表明，L-精氨酸的作用持续时间相当短，并且与地西泮具有相加的抗惊厥作用。
