Yamalik N, Cağlayan F, Kilinç K, Kilinç A, Tümer C
Department of Periodontology, Faculty of Dentistry, University of Hacettepe, Ankara, Turkey.
J Periodontol. 2000 Mar;71(3):460-7. doi: 10.1902/jop.2000.71.3.460.
The enzymatic profile of gingival crevicular fluid (GCF) is being analyzed with increasing interest, but related studies lack a general consensus on most methodological points, including the appropriate mode of data presentation.
GCF myeloperoxidase (MPO) and elastase-like activity (ELA) levels were spectrophotometrically determined on a total of 60 subjects who were divided into three equal subgroups as early-onset periodontitis (EOP), adult periodontitis (AP), and healthy. GCF enzyme levels were calculated and evaluated both as total enzyme activity and enzyme concentration. The correlations between these GCF enzymes and clinical periodontal status were also analyzed.
With both modes of data presentation, the results regarding MPO activity were consistent. When presented either as total MPO activity or MPO concentration, the periodontally healthy group showed significantly lower MPO activity than the two patient groups (P<0.05). However, two modes of data presentation did not match when GCF ELA was concerned. When data were reported as total ELA, the healthy group exhibited lower enzyme activity (0.02 +/- 0.001 U) than EOP (0.04 +/- 0.01 U) and AP (0.06 +/- 0.02 U) groups; but when reported as concentration, the highest ELA levels were seen in the healthy group (221 +/- 31.53 nmol/min/ml), followed by AP (98.63 +/- 23.03 nmol/min/ml) and EOP (70.49 +/- 12.02 nmol/min/ml) (P<0.05). A strong-positive and significant correlation existed between GCF MPO and ELA. Correlations with clinical parameters were mostly observed with total activities.
The findings of the present study confirm the relationship between GCF ELA and MPO activity and periodontal disease and also support the functional relativity between the two enzymes. Furthermore, based on these findings, it can be suggested that data presentation by use of total activity seems to be more sensitive in both the reflection of the actual enzymatic profile of GCF and also the existing clinical periodontal status. For each GCF component, the validity of different modes of data presentation should be considered.
龈沟液（GCF）的酶谱分析正受到越来越多的关注，但相关研究在大多数方法学要点上缺乏普遍共识，包括数据呈现的适当方式。
采用分光光度法测定了总共60名受试者的GCF髓过氧化物酶（MPO）和类弹性蛋白酶活性（ELA）水平，这些受试者被平均分为三个亚组，即早发性牙周炎（EOP）组、成人牙周炎（AP）组和健康组。GCF酶水平以总酶活性和酶浓度两种方式进行计算和评估。还分析了这些GCF酶与临床牙周状况之间的相关性。
两种数据呈现方式下，关于MPO活性的结果是一致的。无论是以总MPO活性还是MPO浓度表示，牙周健康组的MPO活性均显著低于两个患者组（P<0.05）。然而，在GCF ELA方面，两种数据呈现方式并不一致。当以总ELA报告数据时，健康组的酶活性（0.02±0.001 U）低于EOP组（0.04±0.01 U）和AP组（0.06±0.02 U）；但以浓度报告时，健康组的ELA水平最高（221±31.53 nmol/min/ml），其次是AP组（98.63±23.03 nmol/min/ml）和EOP组（70.49±12.02 nmol/min/ml）（P<0.05）。GCF MPO和ELA之间存在强阳性且显著的相关性。与临床参数的相关性大多在总活性方面观察到。
本研究结果证实了GCF ELA和MPO活性与牙周疾病之间的关系，也支持了这两种酶之间的功能相关性。此外，基于这些发现，可以认为使用总活性进行数据呈现似乎在反映GCF的实际酶谱和现有临床牙周状况方面都更敏感。对于每个GCF成分，应考虑不同数据呈现方式的有效性。
