Jones R D, Stuart B P, Greufe N P, Landes A M
Bayer Corporation, Toxicology Department, Stilwell, Kansas 66085-9104, USA.
Lab Anim. 1999 Oct;33(4):356-65. doi: 10.1258/002367799780487832.
Six male and six female Yucatan pigs were utilized to investigate the feasibility of this species as a non-rodent model for routine regulatory and mechanistic toxicology studies. This study evaluated disease surveillance and computerized electrophysiology, along with possible gross and micropathology changes. Two pigs were used as sentinel animals to evaluate the microbiological status of the vendor upon arrival; the other pigs were maintained as biomonitors and to provide baseline clinical chemistry, urinalysis, pathology and electrophysiology data. The electrophysiology tests conducted included electrocardiography (ECG), electroretinography (ERG) and quantitative electroencephalography (qEEG), which achieved consistent baseline values with acceptable intrasubject variation. Tissue cholinesterase and histochemical staining were done to determine their suitability for testing cholinesterase compounds. Evaluation of the serum chemistry profile demonstrated increased CPK and LDH, which was likely associated with slight haemolysis or minor subclinical muscle stress during handling. There were no additional clinical chemistry changes or findings in haematology, urinalysis parameters or gross pathology. Micropathology found an absence of background lesions which would interfere with routine toxicology studies, except for a mild rhinitis. The aetiological agent was identified by electron microscopy as being consistent with inclusion body rhinitis of swine, previously unreported in miniature swine. This would most notably interfere with inhalation studies. The anatomical and physiological similarities of the Yucatan pig, along with its ability to accept the performance of electrophysiology tests allow this species to be considered as a suitable model for organ system testing in toxicology studies.
选用6只雄性和6只雌性尤卡坦猪，以研究该物种作为常规监管和机制毒理学研究的非啮齿动物模型的可行性。本研究评估了疾病监测和计算机化电生理学，以及可能的大体和微观病理学变化。使用2只猪作为哨兵动物，在到达时评估供应商的微生物学状况；其他猪作为生物监测器饲养，并提供基线临床化学、尿液分析、病理学和电生理学数据。进行的电生理学测试包括心电图（ECG）、视网膜电图（ERG）和定量脑电图（qEEG），这些测试获得了一致的基线值，受试者内变异可接受。进行组织胆碱酯酶和组织化学染色以确定它们对胆碱酯酶化合物测试的适用性。血清化学谱评估显示肌酸磷酸激酶（CPK）和乳酸脱氢酶（LDH）升高，这可能与处理过程中的轻微溶血或轻微亚临床肌肉应激有关。血液学、尿液分析参数或大体病理学方面没有其他临床化学变化或发现。微观病理学发现除轻度鼻炎外，没有会干扰常规毒理学研究的背景病变。通过电子显微镜鉴定病原体与猪包涵体鼻炎一致，此前在小型猪中未报道过。这最明显会干扰吸入研究。尤卡坦猪的解剖学和生理学相似性，以及其接受电生理学测试的能力，使得该物种可被视为毒理学研究中器官系统测试的合适模型。
