Arrieta I, del Barrio M, Vidarte L, del Pozo V, Pastor C, Gonzalez-Cabrero J, Cárdaba B, Rojo M, Mínguez A, Cortegano I, Gallardo S, Aceituno E, Palomino P, Vivanco F, Lahoz C
Immunology Department, Fundación Jiménez Díaz, 28040, Madrid, Spain.
Mol Biochem Parasitol. 2000 Apr 15;107(2):263-8. doi: 10.1016/s0166-6851(00)00192-4.
Ingestion of the parasitic nematode Anisakis simplex in undercooked fish can cause severe allergic reactions in some individuals. Using pooled human sera from sensitized patients we have probed an expression library for A. simplex antigens. One positive clone was found to encode a full length 21 kDa protein with strong homology to nematode troponins. The recombinant protein was expressed as a GST-fusion protein and found by immunoblot analysis to react with sera from 20% of allergic patients. The presence of functional EF-hand Ca(2+) binding motifs was demonstrated by gel-shift analysis.
食用未煮熟鱼类中的寄生线虫简单异尖线虫会在一些个体中引发严重过敏反应。我们使用来自致敏患者的混合人血清探测了简单异尖线虫抗原的表达文库。发现一个阳性克隆编码一种与线虫肌钙蛋白有高度同源性的全长21 kDa蛋白。该重组蛋白表达为谷胱甘肽S-转移酶（GST）融合蛋白，经免疫印迹分析发现它与20%的过敏患者血清发生反应。凝胶迁移分析证实了功能性EF-手型钙离子结合基序的存在。
