Lee Y H, Lee H Y, Kim H R
Department of Biology, Korea University, Seoul, South Korea.
Comp Biochem Physiol A Mol Integr Physiol. 2000 Jan;125(1):95-104. doi: 10.1016/s1095-6433(99)00164-6.
Epidermis-origin hemolymph protein (EOHP) was identified and purified from the last instar larval hemolymph of Galleria mellonella by anion exchange chromatography, chromatofocusing chromatography, and Sephadex G-100. The EOHP has a native molecular mass of 47 kDa and is composed of one subunit. The isoelectric point of the EOHP was determined to be 5.3. The amino acid composition of the EOHP was rich in aspartic acid, glutamic acid and lysine, but poor in tyrosine, methionine, and tryptophan. EOHP is present in hemolymph over the period from the 4th instar larvae to the adult stage examined. Concentration of EOHP is high during the larval stage but gradually decreased during the developmental stage from pupal to adult stage. EOHP is present in the cuticle, fat bodies and trachea but not in hemocytes, fore gut, mid gut and hind gut.
通过阴离子交换色谱法、聚焦色谱法和葡聚糖凝胶G - 100，从大蜡螟末龄幼虫的血淋巴中鉴定并纯化出表皮来源的血淋巴蛋白（EOHP）。EOHP的天然分子量为47 kDa，由一个亚基组成。EOHP的等电点测定为5.3。EOHP的氨基酸组成富含天冬氨酸、谷氨酸和赖氨酸，但酪氨酸、蛋氨酸和色氨酸含量较低。在所检测的从四龄幼虫到成虫阶段，血淋巴中均存在EOHP。EOHP在幼虫阶段浓度较高，但在从蛹到成虫的发育阶段逐渐降低。EOHP存在于表皮、脂肪体和气管中，但不存在于血细胞、前肠、中肠和后肠中。
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