L-2'-脱氧鸟苷5'-三磷酸（L-dGTP）和L-2'-脱氧胸苷5'-三磷酸（L-dTTP）对人端粒酶的抑制作用。

Inhibitory effects of of L-2'-deoxyguanosine 5'-triphosphate (L-dGTP) and L-2'-deoxythymidine 5'-triphosphate (L-dTTP) on human telomerase.

作者信息

Yamaguchi T, Yamada R, Shudo K, Saito M, Ishikawa F, Saneyoshi M

机构信息

Department of Biological Sciences, Teikyo University of Science and Technology, Uenohara, Yamanashi, Japan.

出版信息

Nucleic Acids Symp Ser. 1999(42):205-6. doi: 10.1093/nass/42.1.205.

DOI:10.1093/nass/42.1.205

PMID:10780451

Abstract

Telomerase, which synthesizes telomeric DNA in eukaryotic cells, is classified as a reverse transcriptase. To clarify the recognition of 2'-deoxyribonucleoside 5'-triphosphate (dNTP) chirality by telomerase, we studied the inhibitory effects of L-dGTP on HeLa cell telomerase activity using a quantitative 'stretch PCR' assay. L-dGTP had a weakly inhibitory effect (IC50 = 200 microM) in the presence of 10 microM dGTP. This effect was less obvious when the concentration of dGTP was higher. L-dTTP had a similar inhibitory effect. These findings suggest that telomerase may bind to L-dGTP and L-dTTP, and that the ability of telomerase to bind to dGTP or dTTP is changed.

摘要

端粒酶可在真核细胞中合成端粒DNA，被归类为逆转录酶。为阐明端粒酶对2'-脱氧核糖核苷5'-三磷酸（dNTP）手性的识别，我们使用定量“延伸PCR”分析法研究了L-dGTP对HeLa细胞端粒酶活性的抑制作用。在存在10 microM dGTP的情况下，L-dGTP具有微弱的抑制作用（IC50 = 200 microM）。当dGTP浓度较高时，这种作用不太明显。L-dTTP具有类似的抑制作用。这些发现表明，端粒酶可能与L-dGTP和L-dTTP结合，并且端粒酶与dGTP或dTTP结合的能力发生了变化。