Imaging the RecA-DNA complex by atomic force microscopy.
作者信息
Umemura K, Ikawa S, Nishinaka T, Shibata T, Kuroda R
机构信息
Joint Research Center for Atom Technology, Tsukuba, Ibaraki, Japan.
出版信息
Nucleic Acids Symp Ser. 1999(42):235-6. doi: 10.1093/nass/42.1.235.
PMID:10780466
Abstract
Atomic force microscopy (AFM) was applied to study the RecA protein and its complexes with DNA in air and in aqueous solution. RecA and DNA were reacted under several conditions, and deposited onto a mica substrate pre-treated in various ways. We found that the structure of the RecA and RecA-DNA complexes, especially the height of the molecules, was affected by the sample preparation method such as gel filtration, and environment during imaging.
摘要
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