Kuwahara J, Azumano M, Takeda T
Institute for Medicinal Resources, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Nucleic Acids Symp Ser. 1999(42):293-4. doi: 10.1093/nass/42.1.293.
Transcription factor Sp1 is importantly related to expression of many cellular genes. In order to identify the nuclear localization signal of Sp1, various truncated regions of Sp1 were fused to green fluorescent protein (GFP) and were expressed in HeLa cell. The C-terminal region are required to localize Sp1 in the nucleus of HeLa cell.
转录因子Sp1与许多细胞基因的表达密切相关。为了确定Sp1的核定位信号，将Sp1的各个截短区域与绿色荧光蛋白（GFP）融合，并在HeLa细胞中表达。C末端区域是Sp1定位于HeLa细胞核所必需的。
