Potter P C, Van Wyk L, Martin M, Lentes K U, Dowdle E B
Department of Clinical Science and Immunology, University of Cape Town, South Africa.
Clin Exp Allergy. 1993 Oct;23(10):874-7. doi: 10.1111/j.1365-2222.1993.tb00267.x.
To investigate a possible genetic basis for reported differences in beta-2 receptor expression in atopic subjects, DNA from 42 atopic children (22 asthmatics and 22 with allergic rhinitis) and 30 non-atopic subjects was Southern blotted and Ban-1 restriction fragment polymorphisms (RFLPS) were studied using a 2.6 kb probe of the human beta-2 receptor gene. Two alleles 3.1 kb and 2.9 kb were identified. Homozygotes and heterozygotes for the two alleles were found with equal frequency in the atopic patients who had asthma and in those who had allergic rhinitis only. The gene frequencies for the upper and lower alleles were 0.45 and 0.55 respectively. Our studies do not provide evidence for an association between a particular polymorphic form of the human beta-2 receptor gene and atopy.
为了研究特应性个体中β-2受体表达差异可能的遗传基础，对42名特应性儿童（22名哮喘患者和22名变应性鼻炎患者）及30名非特应性个体的DNA进行了Southern印迹分析，并使用人β-2受体基因的2.6 kb探针研究了Ban-1限制性片段多态性（RFLPS）。鉴定出两个等位基因，分别为3.1 kb和2.9 kb。在患有哮喘的特应性患者和仅患有变应性鼻炎的患者中，发现这两个等位基因的纯合子和杂合子频率相等。上、下等位基因的基因频率分别为0.45和0.55。我们的研究未提供证据表明人β-2受体基因的特定多态形式与特应性之间存在关联。
