Ichii S, Imai Y, Irimura T
Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
Cancer Immunol Immunother. 2000 Apr;49(1):1-9. doi: 10.1007/s002620050021.
We used histological observations and experiments with fluorescent cell tracers to investigate the roles of tissue macrophages in recognition through a galactose/N-acetylgalactosamine-specific C-type lectin (mMGL) in lymph node metastasis formation by mouse ovarian tumor OV2944-HM-1 (HM-1) cells. Lymph node metastasis from subcutaneous sites was shown to be initiated by the entry of tumor cells into the subcapsular sinus of lymph nodes where mMGL-positive cells were mainly located. To investigate whether mMGL-positive cells contributed to host resistance against lymph node metastasis, we repeatedly treated mice bearing transplanted tumors with an mMGL-blocking monoclonal antibody that was known to inhibit mMGL binding to its ligands. The number of HM-1 cells recovered from lymph nodes 2 weeks after subcutaneous injections was significantly greater when the mice were treated with the blocking anti-mMGL antibody. These results suggested that mMGL-positive macrophages contributed to the host's defense against lymph node metastasis.
我们利用组织学观察和荧光细胞示踪实验，研究组织巨噬细胞通过半乳糖/N-乙酰半乳糖胺特异性C型凝集素（mMGL）在小鼠卵巢肿瘤OV2944-HM-1（HM-1）细胞形成淋巴结转移过程中的识别作用。皮下部位的淋巴结转移表现为肿瘤细胞进入主要分布有mMGL阳性细胞的淋巴结被膜下窦而启动。为了研究mMGL阳性细胞是否有助于宿主抵抗淋巴结转移，我们用一种已知可抑制mMGL与其配体结合的mMGL阻断单克隆抗体反复处理荷移植瘤小鼠。皮下注射后2周，用抗mMGL阻断抗体处理的小鼠从淋巴结中回收的HM-1细胞数量显著更多。这些结果表明，mMGL阳性巨噬细胞有助于宿主抵御淋巴结转移。
