Sawatsubashi M, Yamada T, Fukushima N, Mizokami H, Tokunaga O, Shin T
The Department of Otolaryngology Head and Neck Surgery, Saga Medical School, Japan.
Virchows Arch. 2000 Mar;436(3):243-8. doi: 10.1007/s004280050037.
We investigated the expression of vascular endothelial growth factor (VEGF) and microvascular density in 54 cases of invasive laryngeal squamous cell carcinoma (SCC) and in ten samples of normal laryngeal tissue using immunohistochemistry methods. The study also focused on the distribution of mast cells in and around the SCCs. The microvascular density in laryngeal carcinoma tissue was higher than that in normal tissue (P = 0.02). VEGF was localized in SCCs, stromal cells, endothelial cells, minor salivary glands, and non-cancer epithelium adjacent to the tumor. VEGF expression in the tumor cells was found in 13 of 54 cases (24.1%), whereas mast cells around the carcinomas were VEGF positive in all 54 cases. Staining of VEGF in SCCs was strong in the area of high microvascular density (P = 0.0002). Using a multi-labeling subtraction immunostaining method, VEGF-positive stromal cells were classified mostly as mast cells and, in a few instances, as macrophages. VEGF staining in SCCs was associated with the mast cell count (P = 0.0001). There was no distinct correlation between VEGF expression and pTNM stage of an SCC. In conclusion, the results suggest that VEGF might be an important angiogenic factor in cancer invasion. Laryngeal cancer cells and mast cells may control the angiogenic response by releasing VEGF.
我们采用免疫组化方法，对54例浸润性喉鳞状细胞癌（SCC）及10份正常喉组织样本中的血管内皮生长因子（VEGF）表达及微血管密度进行了研究。该研究还聚焦于SCC内部及周围肥大细胞的分布情况。喉癌组织中的微血管密度高于正常组织（P = 0.02）。VEGF定位于SCC、基质细胞、内皮细胞、小唾液腺以及肿瘤旁的非癌上皮中。在54例病例中，有13例（24.1%）的肿瘤细胞中发现有VEGF表达，而在所有54例病例中，癌周围的肥大细胞VEGF均呈阳性。在微血管密度高的区域，SCC中VEGF染色较强（P = 0.0002）。采用多标记减法免疫染色方法，VEGF阳性基质细胞大多被分类为肥大细胞，少数情况下为巨噬细胞。SCC中VEGF染色与肥大细胞计数相关（P = 0.0001）。VEGF表达与SCC的pTNM分期之间无明显相关性。总之，结果表明VEGF可能是癌症侵袭过程中的一种重要血管生成因子。喉癌细胞和肥大细胞可能通过释放VEGF来控制血管生成反应。
