Kruså M, Torre M, Marina M L
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá, Madrid, Spain.
Anal Chem. 2000 Apr 15;72(8):1814-8. doi: 10.1021/ac990776m.
A reversed-phase high-performance liquid chromatographic method was designed for the quantitation of soya bean proteins in bovine milks. The method consisted of a linear binary gradient, acetonitrile-water-0.1% trifluoroacetic acid, at a flow rate of 1 mL/min and a temperature of 50 degrees C which resulted in a separation time for soya bean proteins of 11 min. Calibration by the external standard method using a soya bean protein isolate as standard was employed, and the method was validated by evaluating precision, accuracy, and robustness. This method was shown to be useful for the analysis of soya bean proteins in bovine milks spiked with soya bean protein isolate; soya bean protein concentrations of approximately 13 microg/g of bovine milk could be detected by using the optimized method. The results obtained for some of the bovine milks were compared with those obtained by the method of standard additions.
设计了一种反相高效液相色谱法用于定量测定牛乳中的大豆蛋白。该方法采用线性二元梯度，乙腈 - 水 - 0.1% 三氟乙酸，流速为1 mL/min，温度为50℃，大豆蛋白的分离时间为11分钟。采用以大豆分离蛋白为标准品的外标法进行校准，并通过评估精密度、准确度和稳健性对该方法进行验证。结果表明该方法可用于分析添加了大豆分离蛋白的牛乳中的大豆蛋白；使用优化后的方法可检测到牛乳中大豆蛋白浓度约为13 μg/g。将部分牛乳获得的结果与标准加入法获得的结果进行了比较。
