Kawamoto S, Ohno K, Tategaki A, Aki T, Shigeta S, Jyo T, Suzuki O, Ono K
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.
Immunol Lett. 2000 Apr 3;72(1):53-60. doi: 10.1016/s0165-2478(00)00163-2.
Here we describe the detection of T-cell epitope region on the house dust mite allergen Mag 3, which has been shown to trigger T-cell proliferation in mite-allergic asthmatic patients. We first examined murine T-cell epitope using T-cell fraction prepared from recombinant Mag 3 (r-Mag 3)-primed H-2k mice. Initial proliferation assay with truncated r-Mag 3 indicated that N-terminal 113 amino acid region was required for triggering T-cell activation. Subsequent epitope scanning with synthetic overlapping peptides revealed that T-cell reactive region was assigned within amino acid range 56-75. We also explored human T-cell determinant using specific T-cells from mite-allergic patients. Intriguingly, we found that amino acid range 56-85, a portion partially overlapping with that identified in r-Mag 3-primed mice, was exclusively recognized by T-cells from different patients. Further investigation of unique T-cell epitope region found in this study would provide insight into the development of animal therapeutic model and/or peptide vaccine for asthma.
在此，我们描述了对屋尘螨变应原Mag 3上T细胞表位区域的检测，该变应原已被证明可在螨过敏性哮喘患者中引发T细胞增殖。我们首先使用从重组Mag 3（r-Mag 3）致敏的H-2k小鼠制备的T细胞组分检测小鼠T细胞表位。对截短的r-Mag 3进行的初始增殖试验表明，触发T细胞活化需要N端113个氨基酸区域。随后用合成重叠肽进行表位扫描显示，T细胞反应区域位于氨基酸范围56 - 75内。我们还使用来自螨过敏性患者的特异性T细胞探索了人类T细胞决定簇。有趣的是，我们发现氨基酸范围56 - 85，这部分与在r-Mag 3致敏小鼠中鉴定出的区域部分重叠，被来自不同患者的T细胞特异性识别。对本研究中发现的独特T细胞表位区域的进一步研究将为哮喘动物治疗模型和/或肽疫苗的开发提供见解。
