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[DNA与膜的附着在耐辐射微球菌辐射损伤修复中的作用]

[Role of DNA-membrane attachment in repair of radiation damage in Micrococcus radiodurans].

作者信息

Dardalhon-Samsonoff M, Rebeyrotte N

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Feb;27(2):157-69. doi: 10.1080/09553007514550161.

DOI:10.1080/09553007514550161
PMID:1079020
Abstract

The unirradiated bacterial DNA assciated with the membrane is liberated into the cytoplasm after breakage of either a single or a double strand, resulting from X-ray action. During the reincubation period in growth-medium, the DNA is reassociated with the membrane. This phenomenon is very rapid and occurs without increasing the molecular weight of DNA. The study of DNA-membrane complexes shows that the size of the DNA-associated membranous fragment differs according to the lysing technique employed, appearing as a change in the density of the complex. Chloramphenicol decreases reassociation, and iodoacetamide, a radiosensititzing agent, inhibits it completely.

摘要

与细胞膜相关的未受辐射的细菌DNA,在因X射线作用导致单链或双链断裂后会释放到细胞质中。在生长培养基中再次孵育期间,DNA会重新与细胞膜结合。这种现象非常迅速,且在不增加DNA分子量的情况下发生。对DNA-细胞膜复合物的研究表明,与DNA相关的膜片段大小会因所采用的裂解技术而异,表现为复合物密度的变化。氯霉素会降低重新结合的程度,而放射增敏剂碘乙酰胺则会完全抑制它。

相似文献

1
[Role of DNA-membrane attachment in repair of radiation damage in Micrococcus radiodurans].[DNA与膜的附着在耐辐射微球菌辐射损伤修复中的作用]
Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Feb;27(2):157-69. doi: 10.1080/09553007514550161.
2
Repair of double-strand breaks in Micrococcus radiodurans.耐辐射微球菌中双链断裂的修复
Basic Life Sci. 1975;5B:507-12. doi: 10.1007/978-1-4684-2898-8_13.
3
The release of a deoxyribonucleic acid fragment after x-irradiation of Micrococcus radiodurans.耐辐射微球菌经X射线照射后脱氧核糖核酸片段的释放
Int J Radiat Biol Relat Stud Phys Chem Med. 1974 Apr;25(4):329-37. doi: 10.1080/09553007414550401.
4
Repair of x-ray damage to the DNA in Micrococcus radiodurans: the effect of 5-bromodeoxyuridine.耐辐射微球菌中X射线对DNA损伤的修复:5-溴脱氧尿苷的作用
J Mol Biol. 1970 Mar;48(3):395-408. doi: 10.1016/0022-2836(70)90053-7.
5
DNA-membrane association and the repair of double breaks in x-irradiated Micrococcus radiodurans.X射线辐照的耐辐射微球菌中DNA与膜的结合及双链断裂的修复
Biochim Biophys Acta. 1971 Sep 30;247(1):38-53. doi: 10.1016/0005-2787(71)90805-7.
6
Demonstration of two types of DNA repair in X-irradiated Micrococcus radiodurans.在经X射线照射的耐辐射微球菌中两种DNA修复类型的证明。
Can J Microbiol. 1971 Apr;17(4):495-9. doi: 10.1139/m71-082.
7
Possibility of the repair of double-strand scissions in Micrococcus radiodurans DNA caused by gamma-rays.耐辐射微球菌DNA中由γ射线引起的双链断裂的修复可能性。
Biochem Biophys Res Commun. 1968 Nov 8;33(3):418-22. doi: 10.1016/0006-291x(68)90588-3.
8
Sensitization to radiation by loss of recombination ability in a temperature-sensitive DNA mutant of Micrococcus radiodurans held at its restrictive temperature.在限制温度下保存的耐辐射微球菌温度敏感型DNA突变体中,由于重组能力丧失而导致对辐射敏感。
J Gen Microbiol. 1972 Sep;72(2):329-38. doi: 10.1099/00221287-72-2-329.
9
Repair of strand breaks in gamma-irradiated Micrococcus radiodurans.γ射线辐照的耐辐射微球菌中链断裂的修复
Int J Radiat Biol Relat Stud Phys Chem Med. 1972 Sep;22(3):301-6. doi: 10.1080/09553007214551091.
10
Involvement of a recombination repair function in disciplined cell division of Micrococcus radiodurans.重组修复功能参与耐辐射微球菌的有序细胞分裂。
J Gen Microbiol. 1975 Feb;86(2):343-57. doi: 10.1099/00221287-86-2-343.

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