Fukai T, Kuroda J, Nomura T
Faculty of Pharmaceutical Sciences, Toho University, Chiba, Japan.
J Am Soc Mass Spectrom. 2000 May;11(5):458-63. doi: 10.1016/S1044-0305(00)00109-4.
We report the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the accurate measurement of mass of low molecular weight compounds (smaller than 1500 Da), a linear peptide, two types of cyclic depsipeptides, a polyhydroxy-macrocyclic lactone, and two prenylated flavonoids, with delayed extraction in the reflector mode. The performance of the MALDI-TOF instrument was less than those of fast atom bombardment and Fourier-transform ion cyclotron resonance mass spectrometry instruments and insufficient to give acceptable accuracy for literature reporting. Nevertheless, when combined with NMR spectrometry and/or amino acid analysis to give information on the numbers of carbon atoms and index of hydrogen deficiency, MALDI was useful for determination of the elemental composition of the low molecular weight compounds available in small quantities.
我们报道了基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）在反射模式下采用延迟提取技术对低分子量化合物（小于1500 Da）、一种线性肽、两种环缩肽、一种多羟基大环内酯以及两种异戊烯基黄酮进行精确质量测量的应用。该MALDI-TOF仪器的性能不如快原子轰击质谱仪和傅里叶变换离子回旋共振质谱仪，不足以给出可用于文献报道的可接受精度。然而，当与核磁共振光谱法和/或氨基酸分析相结合以提供碳原子数和氢缺指数信息时，MALDI对于测定少量可得的低分子量化合物的元素组成是有用的。
