MacHida U, Tojo A, Takahashi S, Iseki T, Ooi J, Nagayama H, Shirafuji N, Mori S, Wada Y, Ogami K, Yamada Y, Sakamaki H, Maekawa T, Tani K, Asano S
Department of Haematology/Oncology, The Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Br J Haematol. 2000 Mar;108(4):747-53. doi: 10.1046/j.1365-2141.2000.01910.x.
To investigate whether granulocyte colony-stimulating factor (G-CSF) administration to donors before harvest may lighten the burden imposed on them and accelerate the bone marrow (BM) recovery, we administered 2 microgram/kg/d of G-CSF for five consecutive days before the marrow harvest. All of the donors tolerated the G-CSF administration well without severe adverse events. After 5 d of G-CSF treatment, CD34+ cells and granulocyte-macrophage colony-forming units (GM-CFU) in the donors' BM exceeded baseline values by 4.2-fold (range 0.71-316) and 1.6-fold (0.28-118) respectively. The concentration of total nucleated cells (x 107/ml) in the graft increased from 1.61 (0.95-3.23) to 2.44 (1.27-4.01). Although we collected 1020 ml of BM and obtained 1.50 x 1010 nucleated cells from unprimed donors, 940 ml of BM were sufficient to obtain 2.14 x 1010 nucleated cells from primed donors. However, G-CSF-primed BM did not shorten the time to tri-lineage engraftment and the duration of hospitalization compared with unprimed BM, although primed BM contained more CD34+ cells than baseline values. We consider that the advantages of BM priming are not the acceleration of BM recovery but rather the reduction of blood loss during BM harvesting.
为了研究在采集前给供体注射粒细胞集落刺激因子（G-CSF）是否可以减轻他们的负担并加速骨髓（BM）恢复，我们在骨髓采集前连续五天给予2微克/千克/天的G-CSF。所有供体对G-CSF注射耐受性良好，无严重不良事件。G-CSF治疗5天后，供体骨髓中的CD34+细胞和粒细胞-巨噬细胞集落形成单位（GM-CFU）分别比基线值高出4.2倍（范围0.71-316）和1.6倍（0.28-118）。移植物中总核细胞浓度（×107/ml）从1.61（0.95-3.23）增加到2.44（1.27-4.01）。虽然我们从未经预处理的供体中采集了1020毫升骨髓并获得了1.50×1010个有核细胞，但940毫升骨髓足以从经预处理的供体中获得2.14×1010个有核细胞。然而，与未经预处理的骨髓相比，经G-CSF预处理的骨髓并没有缩短三系造血重建时间和住院时间，尽管预处理的骨髓中CD34+细胞比基线值更多。我们认为骨髓预处理的优势不是加速骨髓恢复，而是减少骨髓采集过程中的失血。
