Bull D A, Connors R C, Reid B B, Albanil A, Stringham J C, Karwande S V
Division of Cardiothoracic Surgery, Department of Surgery, University of Utah, 50 North Medical Drive, Salt Lake City, Utah 84132, USA.
J Surg Res. 2000 May 15;90(2):144-8. doi: 10.1006/jsre.2000.5870.
Development of lung preservation solutions typically requires whole-organ models which are animal and labor intensive. These models rely on physiologic rather than biochemical endpoints, making accurate comparison of the relative efficacy of individual solution components difficult. We hypothesized that lung slices could be used to assess preservation of biochemical function during cold storage.
Whole rat lungs were precision cut into slices with a thickness of 500 microm and preserved at 4 degrees C in the following solutions: University of Wisconsin (UW), Euro-Collins (EC), low-potassium-dextran (LPD), Kyoto (K), normal saline (NS), or a novel lung preservation solution (NPS) developed using this model. Lung biochemical function was assessed by ATP content (etamol ATP/mg wet wt) and capacity for protein synthesis (cpm/mg protein) immediately following slicing (0 h) and at 6, 12, 18, and 24 h of cold storage. Six slices were assayed at each time point for each solution. The data were analyzed using analysis of variance and are presented as means +/- SD.
ATP content was significantly higher in the lung slices stored in NPS compared with all other solutions at each time point (P < 0.0001). Protein synthesis was significantly higher in the lung slices stored in NPS compared with all other solutions at 6, 12, and 18 h of preservation (P < 0.05).
This lung slice model allows the rapid and efficient screening of lung preservation solutions and their components using quantifiable biochemical endpoints. Using this model, we have developed a novel solution that improves the biochemical preservation of lung slices during cold storage.
肺保存液的研发通常需要全器官模型，这类模型需要动物且耗费人力。这些模型依赖生理而非生化终点，使得准确比较各个溶液成分的相对功效变得困难。我们推测肺切片可用于评估冷藏期间生化功能的保存情况。
将大鼠全肺精确切成厚度为500微米的切片，并在4℃下保存在以下溶液中：威斯康星大学（UW）溶液、欧洲柯林斯（EC）溶液、低钾右旋糖酐（LPD）溶液、京都（K）溶液、生理盐水（NS）或使用该模型研发的新型肺保存液（NPS）。在切片后立即（0小时）以及冷藏6、12、18和24小时后，通过ATP含量（每毫克湿重的微摩尔ATP）和蛋白质合成能力（每毫克蛋白质的每分钟计数）评估肺的生化功能。每种溶液在每个时间点检测6个切片。数据采用方差分析进行分析，并以平均值±标准差表示。
在每个时间点，与所有其他溶液相比，保存在NPS中的肺切片的ATP含量显著更高（P < 0.0001）。在保存6、12和18小时时，与所有其他溶液相比，保存在NPS中的肺切片的蛋白质合成显著更高（P < 0.05）。
这种肺切片模型允许使用可量化的生化终点快速有效地筛选肺保存液及其成分。利用该模型，我们研发出了一种新型溶液，可改善冷藏期间肺切片的生化保存情况。
