Takamura Y, Sugimoto Y, Kubo E, Takahashi Y, Akagi Y
Department of Ophthalmology, Fukui Medical University, Japan.
Nippon Ganka Gakkai Zasshi. 2000 Apr;104(4):221-5.
To evaluate apoptosis of lens epithelial cells with immunohistochemical methods.
We performed terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assays on capsulotomy specimens (68 eyes in 53 patients) from patients who had undergone cataract surgery and an epithelium of diabetic cataracts in rats (144 eyes in 72 rats). The animal model of diabetic cataracts was prepared by injection of streptozotocin in three-week old rats. The rats were also examined using the proliferating cell nuclear antigen (PCNA) immunohistochemical staining method.
Although some TUNEL-positive cells were detected in capsulotomy specimens, we recognized little correlation between its distribution and morphological classification of cataracts. In the animal model of diabetic cataracts, TUNEL-positive cells were seen around the region where epithelial cells had accumulated. In the accumulated region, PCNA labeled cells undergoing DNA synthesis were also detected.
These results suggest the possibility that apoptosis occurs in human lens epithelial cells and apoptosis and proliferation may be induced by factors such as hyperglycemia.
采用免疫组化方法评估晶状体上皮细胞的凋亡情况。
我们对接受白内障手术患者的囊膜切开术标本（53例患者共68只眼）以及大鼠糖尿病性白内障的晶状体上皮（72只大鼠共144只眼）进行了末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记（TUNEL）检测。糖尿病性白内障动物模型通过向三周龄大鼠注射链脲佐菌素制备。同时采用增殖细胞核抗原（PCNA）免疫组化染色方法对大鼠进行检测。
虽然在囊膜切开术标本中检测到了一些TUNEL阳性细胞，但我们发现其分布与白内障的形态学分类之间几乎没有相关性。在糖尿病性白内障动物模型中，TUNEL阳性细胞出现在上皮细胞积聚的区域周围。在积聚区域，还检测到了进行DNA合成的PCNA标记细胞。
这些结果提示人类晶状体上皮细胞可能发生凋亡，并且高血糖等因素可能诱导凋亡和增殖。
