质子化导致的聚腺苷酸的结构转变：单链长度对双螺旋形式极谱行为的影响。
Structural transitions of polyadenylic acid due to protonation: the influence of the length of single strands on the polarographic behaviour of the double-helical form.
作者信息
Palecek E, Vetterl V, Sponar J
出版信息
Nucleic Acids Res. 1974 Mar;1(3):427-42. doi: 10.1093/nar/1.3.427.
Transition of single-stranded poly(A) into its double-helical protonated form was followed by means of derivative pulse polarography, spectrophotometry, and other methods. It was found that properties of protonated poly(A) depended on the length of single strands from which the protonated double helix was formed. In contrary to longer poly(A) transition of short single-stranded molecules (s(20),w lower than about 3) caused practically no decrease in the pulse-polarographic current. It was concluded that the formation of the protonated double helix of poly(A) did not result in the inaccesibility of the reduction sites (located in the vicinity of the surface of the molecule) for the electrode process, as it was in DNA-like double-helical polynucleotides. The current changes observed in the course of transition of longer poly(A) were explained as due to slower transport of long double-stranded molecules to the electrode.
通过导数脉冲极谱法、分光光度法及其他方法跟踪单链聚腺苷酸（poly(A)）向其双螺旋质子化形式的转变。研究发现，质子化聚腺苷酸的性质取决于形成质子化双螺旋的单链长度。与较长的聚腺苷酸相反，短单链分子（s(20),w低于约3）的转变实际上并未导致脉冲极谱电流降低。得出的结论是，聚腺苷酸质子化双螺旋的形成不会像在类DNA双螺旋多核苷酸中那样导致还原位点（位于分子表面附近）对于电极过程不可接近。较长聚腺苷酸转变过程中观察到的电流变化被解释为是由于长双链分子向电极的传输较慢。
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