枯草芽孢杆菌中核苷的转运：胞苷摄取的特征
Transport of nucleosides in Bacillus subtilis: characteristics of cytidine-uptake.
作者信息
Kloudová A, Fucik V
出版信息
Nucleic Acids Res. 1974 Apr;1(4):629-37. doi: 10.1093/nar/1.4.629.
Abstract
In Bacillus subtilis SMYW cytidine and uridine are transported by a common system. Transport of these substances requires metabolic energy. After 60 sec of (3)II-cytidine-uptake practically all accumulated radioactivity was found in phosphorylated products. Addition of ribonucleosides with inhibitory effect upon cytidine-uptake resulted in competitive type of inhibition while interference with deoxyribonucleosides was of hyperbolic competitive type. Adenosine possesses a high affinity to cytidine-transporting site but requires different system(s) for its own transport. Guanosine, adenine, cytosine and 5-nucleotides do not interfere with cytidine-uptake.
摘要
在枯草芽孢杆菌SMYW中，胞苷和尿苷通过一个共同的系统进行转运。这些物质的转运需要代谢能量。在（3）II-胞苷摄取60秒后，几乎所有积累的放射性都出现在磷酸化产物中。添加对胞苷摄取有抑制作用的核糖核苷会导致竞争性抑制，而脱氧核糖核苷的干扰则是双曲线竞争性抑制类型。腺苷对胞苷转运位点具有高亲和力，但自身转运需要不同的系统。鸟苷、腺嘌呤、胞嘧啶和5-核苷酸不干扰胞苷摄取。
相似文献
1
Transport of nucleosides in Bacillus subtilis: characteristics of cytidine-uptake.枯草芽孢杆菌中核苷的转运：胞苷摄取的特征
Nucleic Acids Res. 1974 Apr;1(4):629-37. doi: 10.1093/nar/1.4.629.
2
Transport of nucleosides in Bacillus subtilis: the effect of purine nucleosides on the cytidine-uptake.枯草芽孢杆菌中核苷的转运：嘌呤核苷对胞苷摄取的影响。
Nucleic Acids Res. 1974 Apr;1(4):639-44. doi: 10.1093/nar/1.4.639.
3
Uptake, transport and metabolism of exogenous nucleosides in intestinal epithelial cell cultures.肠道上皮细胞培养物中外源核苷的摄取、转运和代谢
J Nutr. 1994 Oct;124(10):1942-9. doi: 10.1093/jn/124.10.1942.
4
Uridine and cytidine transport in Escherichia coli B and transport-deficient mutants.大肠杆菌B及转运缺陷型突变体中的尿苷和胞苷转运
J Biol Chem. 1977 Apr 25;252(8):2492-7.
5
Metabolism of pyrimidine bases and nucleosides in Bacillus subtilis.枯草芽孢杆菌中嘧啶碱基和核苷的代谢
J Bacteriol. 1977 Feb;129(2):574-9. doi: 10.1128/jb.129.2.574-579.1977.
6
N6-(delta2-isopentenyl)adenosine, an inhibitor of cellular transport of uridine and cytidine.N6-（δ2-异戊烯基）腺苷，一种尿苷和胞苷细胞转运抑制剂。
J Cell Physiol. 1975 Oct;86(2 Pt 1):281-91. doi: 10.1002/jcp.1040860212.
7
Endogenous nucleosides in the guinea-pig eye: analysis of transport and metabolites.豚鼠眼中的内源性核苷：转运及代谢产物分析。
Exp Eye Res. 1998 Mar;66(3):315-25. doi: 10.1006/exer.1997.0424.
8
Giardia lamblia: uptake of pyrimidine nucleosides.蓝氏贾第鞭毛虫：嘧啶核苷的摄取
Exp Parasitol. 1987 Apr;63(2):152-6. doi: 10.1016/0014-4894(87)90156-1.
9
Passive diffusion of nucleosides into Micrococcus sodonensis membrane vesicles.核苷被动扩散进入嗜钠微球菌膜囊泡。
Can J Biochem. 1980 Jun;58(6):457-60. doi: 10.1139/o80-060.
10
Profiles of pyrimidine biosynthesis, salvage and degradation in disks of potato (Solanum tuberosum L.) tubers.马铃薯（Solanum tuberosum L.）块茎圆盘嘧啶生物合成、补救和降解的概况。
Planta. 2002 Sep;215(5):821-8. doi: 10.1007/s00425-002-0806-5. Epub 2002 Jun 21.
引用本文的文献
1
A Small Molecule Inhibitor of CTP Synthetase Identified by Differential Activity on a Mutant Deficient in Class A Penicillin-Binding Proteins.通过对A类青霉素结合蛋白缺陷突变体的差异活性鉴定出的CTP合成酶小分子抑制剂。
Front Microbiol. 2020 Aug 26;11:2001. doi: 10.3389/fmicb.2020.02001. eCollection 2020.
2
Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase.枯草芽孢杆菌中编码胞苷三磷酸合成酶的pyrG基因转录的调控
J Bacteriol. 2001 Oct;183(19):5513-22. doi: 10.1128/JB.183.19.5513-5522.2001.
3
Dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein.枯草芽孢杆菌的Dra-nupC-pdp操纵子：核苷酸序列、脱氧核苷诱导作用以及由deoR编码的DeoR阻遏蛋白的转录调控
J Bacteriol. 1996 Jan;178(2):424-34. doi: 10.1128/jb.178.2.424-434.1996.
本文引用的文献
1
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.脱氧核糖核酸对枯草芽孢杆菌生化缺陷菌株的转化
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8. doi: 10.1073/pnas.44.10.1072.
2
The determination of enzyme inhibitor constants.酶抑制剂常数的测定
Biochem J. 1953 Aug;55(1):170-1. doi: 10.1042/bj0550170.
3
The relationship of adenosine and inosine transport in Escherichia coli.大肠杆菌中腺苷和肌苷转运的关系。
Biochim Biophys Acta. 1966 Sep 5;126(1):129-45. doi: 10.1016/0926-6585(66)90043-4.
4
The effect of phenethyl alcohol on Bacillus subtilis transformation. II. Transport of DNA and precursors.苯乙醇对枯草芽孢杆菌转化的影响。II. DNA及前体的转运
Biochim Biophys Acta. 1969 Jan 21;174(1):276-81. doi: 10.1016/0005-2787(69)90251-2.
5
Energy requirements, interactions and distinctions in the mechanisms for transport of various nucleosides in Escherichia coli.大肠杆菌中各种核苷转运机制的能量需求、相互作用及差异
Biochim Biophys Acta. 1967 Sep 9;135(4):771-83. doi: 10.1016/0005-2736(67)90108-3.
6
Kinetic analysis of two component systems in transmembrane transport (multiple forms of transport systems).跨膜运输中双组分系统的动力学分析（多种形式的运输系统）
Biophysik. 1972;8(4):271-9. doi: 10.1007/BF01190612.
7
The use of Dixon plots to study enzyme inhibition.
Biochim Biophys Acta. 1972 Dec 7;289(2):251-3. doi: 10.1016/0005-2744(72)90074-5.
8
Transport alterations in a phosphatidylethanolamine-deficient mutant of Bacillus subtilis.枯草芽孢杆菌磷脂酰乙醇胺缺陷型突变体中的转运变化
J Bacteriol. 1972 Feb;109(2):939-42. doi: 10.1128/jb.109.2.939-942.1972.
9
Adenosine uptake by isolated membrane vesicles from Escherichia coli K-12.来自大肠杆菌K-12的分离膜囊泡对腺苷的摄取。
Biochim Biophys Acta. 1973 Dec 13;330(2):206-21. doi: 10.1016/0005-2736(73)90226-5.
10
Mechanisms of nonelectrolyte transport.非电解质转运机制。
Biochim Biophys Acta. 1973 Sep 10;300(2):183-210. doi: 10.1016/0304-4157(73)90004-x.
Zotero 插件