使用S1核酸酶从琼脂中回收RNA-DNA杂交体进行杂交分离。
Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease.
作者信息
Hansen J N, Pheiffer B H, Hough C J
出版信息
Nucleic Acids Res. 1974 Jun;1(6):787-801. doi: 10.1093/nar/1.6.787.
Abstract
A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.
摘要
描述了一种使用单链特异性核酸酶从琼脂中回收RNA-DNA杂交体的方法。该程序适用于大规模分离，并且在杂交前将DNA固定在琼脂中可实现高产量的杂交体，而不受DNA重新退火的干扰。
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