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使用S1核酸酶从琼脂中回收RNA-DNA杂交体进行杂交分离。

Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease.

作者信息

Hansen J N, Pheiffer B H, Hough C J

出版信息

Nucleic Acids Res. 1974 Jun;1(6):787-801. doi: 10.1093/nar/1.6.787.

DOI:10.1093/nar/1.6.787
PMID:10793757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343380/
Abstract

A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.

摘要

描述了一种使用单链特异性核酸酶从琼脂中回收RNA-DNA杂交体的方法。该程序适用于大规模分离，并且在杂交前将DNA固定在琼脂中可实现高产量的杂交体，而不受DNA重新退火的干扰。

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本文引用的文献

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SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
2
CHROMATOGRAPHIC SEPARATION OF ANNEALED AND ENZYMATICALLY SYNTHESIZED RNA-DNA HYBRIDS.退火及酶促合成的RNA-DNA杂交体的色谱分离
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Isolation of Escherichia coli transfer RNA-gene hybrids.大肠杆菌转移RNA-基因杂种的分离
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Isolation of repetitive DNA from two mammalian species using S1 nuclease.
Biochem Biophys Res Commun. 1974 Jan;56(1):9-13. doi: 10.1016/s0006-291x(74)80308-6.
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Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.米曲霉单链特异性核酸酶的纯化及其他特性
Eur J Biochem. 1973 Feb 15;33(1):192-200. doi: 10.1111/j.1432-1033.1973.tb02669.x.
6
Mung bean nuclease I. V. A tool for probing low melting regions in deoxyribonucleic acid.绿豆核酸酶I. V. 一种探测脱氧核糖核酸中低熔点区域的工具。
J Biol Chem. 1973 Feb 25;248(4):1277-80.
7
Use of exhaustive nucleic acid hybridization for determining the amount and size distribution of newly synthesized bacterial message RNA.使用彻底的核酸杂交法测定新合成的细菌信使核糖核酸的数量和大小分布。
Biochem Genet. 1973 Oct;10(2):175-90. doi: 10.1007/BF00485764.
8
Estimation of the double-helical content in various single-stranded nucleic acids by treatment with a single strand-specific nuclease.通过用单链特异性核酸酶处理来估计各种单链核酸中的双螺旋含量。
Biochim Biophys Acta. 1972 Dec 22;287(3):477-84. doi: 10.1016/0005-2787(72)90292-4.
9
Isolation of a high-molecular-weight hybrid between ribosomal RNA and DNA.核糖体RNA与DNA之间高分子量杂交体的分离
Eur J Biochem. 1972 Jul 24;28(3):389-98. doi: 10.1111/j.1432-1033.1972.tb01925.x.
10
Electron microscope mapping of the distribution of ribosomal genes of the Bacillus subtilis chromosome.枯草芽孢杆菌染色体核糖体基因分布的电子显微镜图谱分析
J Mol Biol. 1973 Apr 5;75(2):265-79. doi: 10.1016/0022-2836(73)90020-x.