Mylotte J M, Tayara A
Department of Medicine, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, USA.
Eur J Clin Microbiol Infect Dis. 2000 Mar;19(3):157-63. doi: 10.1007/s100960050453.
During the past two decades, there have been important advances in blood culture methodology. These advances have resulted in earlier detection and identification of pathogens causing bloodstream infections. However, there are many facets of the blood culture as a diagnostic test that are not affected by new culture methods or systems that continue to cause problems with interpretation of results. The objective of this review is to focus on those factors influencing the results of blood cultures that have clinical relevance. Such factors include skin preparation, timing, procurement techniques, volume of blood obtained, number of cultures, anaerobic blood cultures, and contamination. In addition, bacteremia prediction models are discussed and suggestions are provided as to how these models could be of greater clinical use. Blood culture methods and systems are not discussed in this review.
在过去二十年中，血培养方法取得了重要进展。这些进展使得能够更早地检测和鉴定引起血流感染的病原体。然而，作为一种诊断测试，血培养有许多方面不受新的培养方法或系统的影响，这些方面仍然会导致结果解读出现问题。本综述的目的是关注那些影响血培养结果且具有临床相关性的因素。这些因素包括皮肤准备、采血时间、采血技术、采血量、培养次数、厌氧血培养以及污染情况。此外，还讨论了菌血症预测模型，并就如何使这些模型在临床上发挥更大作用提出了建议。本综述不讨论血培养方法和系统。
