Gunvanti Rathod, Lakshmi Jyothi Tadi, Ariyanachi Kaliappan, Saranya Mallamgunta, Kamlakar Sarvam, Sakthivadivel Varatharajan, Gaur Archana, Nikhat Shalam Shireen, Sagar Triveni, Chenna Kesavulu, Vidya Meena S
Department of Pathology & Lab Medicine, All India Institute of Medical Sciences (AIIMS), Bibinagar, Telangana, India.
Department of Microbiology, All India Institute of Medical Sciences, Bibinagar, India.
Maedica (Bucur). 2022 Jun;17(2):311-316. doi: 10.26574/maedica.2022.17.2.311.
Blood culture test is the gold standard test to diagnose bloodstream infections, but contamination is the main problem in this valuable test. False positive results in blood cultures are mainly due to contamination that occurs mostly during pre-analytical procedures like sample collection and sometimes during sample processing. Our prospective observational study was undertaken at St. Theresa Hospital, Hyderabad, India, during January 2020-June 2020. Blood cultures received from inpatient departments (IPD) and outpatient departments (OPD) are included. The contamination rate was calculated by dividing the total number of contaminated blood cultures by the total number of cultures multiplied by 100. Blood culture contamination rate is 2.4%, which is within the limit as per the standard guideline. Contamination occurred mainly due to improper disinfection of the skin and environmental contamination.
血培养检测是诊断血流感染的金标准检测,但污染是这项重要检测中的主要问题。血培养的假阳性结果主要归因于污染,污染大多发生在诸如样本采集等分析前程序中,有时也发生在样本处理过程中。我们的前瞻性观察性研究于2020年1月至2020年6月在印度海得拉巴的圣特雷莎医院进行。纳入了从住院部(IPD)和门诊部(OPD)采集的血培养样本。污染率通过将污染的血培养总数除以培养总数再乘以100来计算。血培养污染率为2.4%,在标准指南规定的限度内。污染主要是由于皮肤消毒不当和环境污染所致。