Airenne K J, Marjomäki V S, Kulomaa M S
Department of Biological and Environmental Science, University of Jyväskylä, Finland.
Biomol Eng. 1999 Dec 31;16(1-4):87-92. doi: 10.1016/s1050-3862(99)00037-6.
Both chicken egg-white avidin and its bacterial relative streptavidin are well known for their extraordinary high affinity with biotin (Kd approximately 10(-15) M). They are widely used as tools in a number of affinity-based separations, in diagnostic assays and in a variety of other applications. These methods have collectively become known as (strept)avidin-biotin technology. Biotin can easily and effectively be attached to different molecules, termed binders and probes, without destroying their biological activity. The exceptional stability of the avidin-biotin complex and the wide range of commercially available reagents explain the popularity of this system. In order by genetic engineering to modify the unwanted properties of avidin and to further expand the existing avidin-biotin technology, production systems for recombinant avidin and avidin-fusion proteins have been established. This review article presents an overview of the current status of these systems. Future trends in the production and applications of recombinant avidin and avidin-fusion proteins are also discussed.
鸡卵清白蛋白抗生物素蛋白及其细菌同源物链霉抗生物素蛋白都因其与生物素具有极高的亲和力（解离常数Kd约为10⁻¹⁵M）而闻名。它们在许多基于亲和力的分离、诊断分析以及各种其他应用中被广泛用作工具。这些方法统称为（链霉）抗生物素蛋白-生物素技术。生物素能够轻松且有效地连接到不同的分子上，这些分子被称为结合物和探针，同时不会破坏它们的生物活性。抗生物素蛋白-生物素复合物的非凡稳定性以及大量可商购的试剂解释了该系统为何如此受欢迎。为了通过基因工程改造抗生物素蛋白的不良特性并进一步扩展现有的抗生物素蛋白-生物素技术，已建立了重组抗生物素蛋白和抗生物素蛋白融合蛋白的生产系统。这篇综述文章概述了这些系统的当前状况。还讨论了重组抗生物素蛋白和抗生物素蛋白融合蛋白在生产和应用方面的未来趋势。
