60钴 - 乙二胺四乙酸作为蛙皮细胞外标记物的应用。

The use of 60Co-EDTA as an extracellular marker in frog skin.

作者信息

Ferreira K T, Swensson W M

出版信息

Biochim Biophys Acta. 1979 Mar 23;552(1):178-82. doi: 10.1016/0005-2736(79)90256-6.

Abstract

60Co-EDTA was tested as an extracellular marker in isolated epithelia of frog skin. It was found to be non-toxic for frog skin and 0.1 mM EDTA proved to be enough to saturate any adsorption sites. Comparing with [14C]inulin, 60Co-EDTA marks a slightly greater extracellular space and this volume is constant between 15 min and 2 h. Furthermore it is reproducible and its use simplifies the methodology of the determination of water and electrolyte contents in the cells.

摘要

60Co - 乙二胺四乙酸（60Co - EDTA）作为一种细胞外标记物在青蛙皮肤的离体上皮组织中进行了测试。结果发现它对青蛙皮肤无毒，且0.1 mM的乙二胺四乙酸（EDTA）足以饱和任何吸附位点。与[14C]菊粉相比，60Co - EDTA标记的细胞外空间略大，且该体积在15分钟至2小时之间保持恒定。此外，它具有可重复性，其使用简化了细胞内水和电解质含量测定的方法。