Rankin C H, Gannon T, Wicks S R
Dept. of Psychology, University of British Columbia, Vancouver, British Columbia, Canada.
Dev Psychobiol. 2000 May;36(4):261-70.
Habituation and spontaneous recovery from habituation to tap were studied across development in C. elegans. Unlike adult worms, larval worms do not consistently swim backwards to tap, but reverse half of the time and accelerate forward half of the time. In adult worms, the tap response is produced by the integration of two competing circuits: The head touch circuit, mediated by ALM and AVM sensory neurons, produces backward movement (reversals); the tail touch circuit, mediated by PLM neurons, produces forward movement (accelerations). Because the response type changes over development, habituation of each of the subcircuits was studied separately. Habituation of the head touch circuit was studied by laser ablating PLM, and habituation of the tail circuit was studied by ablating ALM. Worms were tested at six stages of development at either 10- or 60-s interstimulus intervals. All stages of development showed normal habituation and spontaneous recovery at both interstimulus intervals.
在秀丽隐杆线虫的整个发育过程中，研究了对轻敲刺激的习惯化以及从习惯化中自发恢复的情况。与成虫不同，幼虫对轻敲刺激并非始终向后游动，而是一半时间向后反转，另一半时间向前加速。在成虫中，轻敲反应由两个相互竞争的神经回路整合产生：由ALM和AVM感觉神经元介导的头部触觉回路产生向后运动（反转）；由PLM神经元介导的尾部触觉回路产生向前运动（加速）。由于反应类型在发育过程中会发生变化，因此分别研究了每个子回路的习惯化情况。通过激光消融PLM来研究头部触觉回路的习惯化，通过消融ALM来研究尾部回路的习惯化。在发育的六个阶段，以10秒或60秒的刺激间隔对蠕虫进行测试。在两个刺激间隔下，所有发育阶段均表现出正常的习惯化和自发恢复。
