Sahu S C, Chou M W, Sotomayor R E, Hinton D M, Barton C N, O'Donnell M W
Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, MD 20708, USA.
J Appl Toxicol. 2000 May-Jun;20(3):215-9. doi: 10.1002/(sici)1099-1263(200005/06)20:3<215::aid-jat649>3.0.co;2-2.
Glutathione S-transferase (GST) plays a major role in the detoxification of the potent hepatocarcinogen aflatoxin B(1) (AFB(1)). This study evaluated the effects of intermittent exposures to AFB(1) on hepatic and testicular GST in rats. Male Fischer 344 rats were fed diets containing AFB(1) (0, 0.01, 0.04, 0.4 and 1.6 ppm) intermittently at 4-week intervals up to 20 weeks. The control animals were fed an AFB(1)-free NIH-31 diet. Rats consuming diets with 0.01 ppm AFB(1) did not show the induction of hepatic or testicular GST activity. Intermittent exposures to AFB(1) at concentrations of 0.04-1.6 ppm significantly increased the GST activities. The increase of the enzyme activity was proportional to the dose and length of AFB(1) exposure.
谷胱甘肽S-转移酶（GST）在强效肝癌致癌物黄曲霉毒素B1（AFB1）的解毒过程中起主要作用。本研究评估了间歇性暴露于AFB1对大鼠肝脏和睾丸中GST的影响。雄性Fischer 344大鼠每隔4周间歇性喂食含AFB1（0、0.01、0.04、0.4和1.6 ppm）的饲料，持续20周。对照动物喂食不含AFB1的NIH-31饲料。食用含0.01 ppm AFB1饲料的大鼠未表现出肝脏或睾丸GST活性的诱导。间歇性暴露于浓度为0.04 - 1.6 ppm的AFB1会显著增加GST活性。酶活性的增加与AFB1暴露的剂量和时长成正比。
