Sakamoto K, Smith G M, Storer P D, Jones K J, Damaser M S
Department of Urology, Loyola University Medical School, Maywood, Illinois, USA.
Neurourol Urodyn. 2000;19(3):311-21. doi: 10.1002/(sici)1520-6777(2000)19:3<311::aid-nau11>3.0.co;2-6.
Since the pudendal nerve innervates the external urethral sphincter, pudendal nerve injury and resultant neuroregeneration should affect voiding behavior. In this study, neuroregenerative activity of pudendal nerve was correlated to the changes in urinary behavior in female rats. Eighteen female rats underwent bilateral pudendal nerve crush, and 17 to 21 age-matched rats were used as unoperated controls. Urinary volume and frequency were recorded 6 and 13 days post-operatively (dpo). Initiation of pudendal nerve regeneration was indicated by an upregulation of beta(II) tubulin mRNA in the dorsolateral motoneurons (DLM), as measured at 7 and 14 dpo by in situ hybridization with radio-labeled beta(II) tubulin cDNA. At 6 dpo, mean volume voided by the crush group was significantly decreased compared to the control group during the light cycle (P < 0.05). At 7 dpo, the DLM mRNA level was significantly increased in the nerve crush group compared to the control group (P < 0.05). At 13 dpo, there were no differences in volume or frequency between the two groups, suggesting a return to normal voiding behavior. At 14 dpo, there was no significant difference in DLM mRNA levels between crush and control groups. Initiation of nerve regeneration occurs before normalization of voiding behavior after pudendal nerve crush. This data suggest that treatments to accelerate nerve regeneration would improve functional recovery of neurologically based incontinence.
由于阴部神经支配尿道外括约肌，阴部神经损伤及由此产生的神经再生应会影响排尿行为。在本研究中，将雌性大鼠阴部神经的神经再生活性与排尿行为的变化相关联。18只雌性大鼠接受双侧阴部神经挤压，17至21只年龄匹配的大鼠用作未手术对照。在术后6天和13天记录尿量和排尿频率。通过用放射性标记的β（II）微管蛋白cDNA进行原位杂交来测量，在术后7天和14天，背外侧运动神经元（DLM）中β（II）微管蛋白mRNA的上调表明阴部神经再生开始。在术后6天，与对照组相比，挤压组在光照周期内的平均排尿量显著减少（P<0.05）。在术后7天，与对照组相比，神经挤压组的DLM mRNA水平显著升高（P<0.05）。在术后13天，两组之间的尿量或排尿频率没有差异，表明排尿行为恢复正常。在术后14天，挤压组和对照组之间的DLM mRNA水平没有显著差异。阴部神经挤压后，神经再生开始于排尿行为恢复正常之前。该数据表明，加速神经再生的治疗将改善神经源性尿失禁的功能恢复。
