Quélo I, Jurdic P
Laboratoire de Biologie Moléculaire et Cellulaire de l'Ecole Normale Supérieure de Lyon, UMR5665 CNRS/ENS, INRA 913, 46, Allée d'Italie, Lyon cédex 07, 69364, France.
Biochem Biophys Res Commun. 2000 May 10;271(2):481-91. doi: 10.1006/bbrc.2000.2654.
The Carbonic Anhydrase II (CAII) gene that encodes an enzyme involved in proton production is expressed in several cell types including monocyte/macrophage-derived osteoclasts. We have analyzed the regulation of the chicken CAII promoter/reporter construct by nuclear hormone receptors of the VDR subfamily in HD11 avian macrophages. The CAII expression is stimulated by 1, 25-dihydroxyvitamin D(3) but not by 9-cis retinoic acid and repressed by VDR overexpression due to RXR squelching. It is also stimulated by all-trans retinoic acid only when RARalpha is overexpressed, and is dependent on a RARE located in the distal part of the promoter and bound by RARalpha homodimer. Finally, in macrophages, unlike in erythrocytes, the CAII promoter is unresponsive to thyroid hormone. Our results demonstrate the first retinoic acid response element in the CAII promoter and show that according to cell type, different nuclear receptors of the VDR subfamily can regulate the CAII gene.
编码参与质子产生的一种酶的碳酸酐酶II（CAII）基因在包括单核细胞/巨噬细胞衍生的破骨细胞在内的几种细胞类型中表达。我们已经在HD11禽巨噬细胞中分析了VDR亚家族的核激素受体对鸡CAII启动子/报告基因构建体的调控。CAII表达受到1,25 - 二羟基维生素D(3)的刺激，但不受9 - 顺式视黄酸的刺激，并且由于RXR的抑制作用，VDR过表达会抑制其表达。仅当RARα过表达时，全反式视黄酸也会刺激其表达，并且其依赖于位于启动子远端并由RARα同二聚体结合的RARE。最后，在巨噬细胞中，与红细胞不同，CAII启动子对甲状腺激素无反应。我们的结果证明了CAII启动子中第一个视黄酸反应元件，并表明根据细胞类型，VDR亚家族的不同核受体可以调节CAII基因。
