一种同时检测和定量T淋巴细胞与B淋巴细胞的方法。

A simultaneous method for detection and quantitation of T- and B-lymphocytes.

作者信息

Robinson J A, Lertratanakul Y

出版信息

J Immunol Methods. 1975;8(1-2):53-60. doi: 10.1016/0022-1759(75)90081-2.

DOI:10.1016/0022-1759(75)90081-2

Abstract

Assay for quantitation of B- and T--lymphocyted from small volumes of heparinized man blood has been developed by simultaneously reacting Ficoll-Hypaque purified mononuclear cells with antobody-coated chicken erythrocytes to for rosettes with B-lymphocytes, and sheep erythrocytes which formed non-immune rosettes with Tlymphocytes. Distribution of various lymphocyte populations in normals are T-cells, 60.9+/-7%, B-cells 16.4+/-4%, and lymphocytes with both receptors, 1.6+/-0.6%.

摘要

已开发出一种从小体积肝素化人血中定量检测B淋巴细胞和T淋巴细胞的方法，该方法是通过使Ficoll-Hypaque纯化的单核细胞与抗体包被的鸡红细胞同时反应以形成B淋巴细胞的玫瑰花结，以及与T淋巴细胞形成非免疫玫瑰花结的绵羊红细胞。正常情况下各种淋巴细胞群体的分布为：T细胞60.9±7%，B细胞16.4±4%，以及具有两种受体的淋巴细胞1.6±0.6%。

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A simultaneous method for detection and quantitation of T- and B-lymphocytes.一种同时检测和定量T淋巴细胞与B淋巴细胞的方法。

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