Lang M A, Caplan S R, Essig A
Biochim Biophys Acta. 1975 Sep 2;401(3):481-5. doi: 10.1016/0005-2736(75)90245-x.
The molting which occurs in frog skin following exposure to high concentrations of aldosterone interferes with the interpretation of physiological measurements. Exposure of skins from frogs maintained in standard smooth tanks to 5 - 10(-7) M aldosterone caused within a few hours erratic responses in short-circuit current Io and conductance K followed by sustained stimulation of Io and K; 10(-8) M aldosterone caused only stimulation of Io and K. Storage of frogs in "rojgh tanks" eliminated in vitro molting on exposure to 5 - 10(-7) M aldosterone. IO and K were then superimposable for 3 h, after which Io increased far more rapidly than K. These results are consistent with an early effect on permeability of the active pathway and later effects on metabolism, either a direct effect on the pump or enhanced interaction between transport and metabolism.
将处于标准光滑水槽中的青蛙皮肤暴露于5 - 10⁻⁷ M醛固酮后发生的蜕皮会干扰生理测量结果的解读。将饲养在标准光滑水槽中的青蛙皮肤暴露于5 - 10⁻⁷ M醛固酮，在数小时内，短路电流Io和电导K会出现不稳定反应，随后Io和K受到持续刺激；10⁻⁸ M醛固酮仅引起Io和K的刺激。将青蛙饲养在“粗糙水槽”中，可消除暴露于5 - 10⁻⁷ M醛固酮时的体外蜕皮现象。然后Io和K在3小时内可叠加，之后Io的增加速度比K快得多。这些结果与早期对主动转运途径通透性的影响以及后期对代谢的影响一致，这种影响要么是对泵的直接作用，要么是转运与代谢之间增强的相互作用。
