Asaumi J, Matsuzaki H, Kawasaki S, Kuroda M, Takeda Y, Kishi K, Hiraki Y
Department of Oral Radiology, Okayama University Dental School, Japan.
Anticancer Res. 2000 Mar-Apr;20(2A):769-72.
In this study, we observed the influence of bovine calf serum (BCS) on the intracellular accumulation of adriamycin (ADR) in Ehrlich ascites tumor cells (wild type EAT cells) and their ADR-resistant strain. The ADR accumulation treated in the medium not containing BCS was 3-fold of that containing 10% BCS. ADR incorporated into cells was not effluxed in the absence of the BCS, while it was rapidly effluxed for 30 minutes to about 20% of the amount incorporated into the cells in the presence of 10% BCS in both strains. This suggests that BCS remarkably decreases the ADR accumulation due to the promotion of ADR efflux. Further, the ADR accumulation decreased with increasing concentrations of BCS in both strains. This indicates that BCS promotes the ADR efflux, depending on its concentrations. BCS was heated at 100 degrees C for 7 minutes and its protein contents were degenerated. Its influence in the ADR accumulation of wild type and ADR-resistant strain was apparent. The ADR accumulation decreased with increasing concentrations of heated BCS in both strains, as well as in the untreated BCS. Thus, the protein content of the BCS did not affect ADR accumulation. Therefore, it is necessary to investigate which components of BCS affect the promotion of ADR efflux.
在本研究中，我们观察了牛犊血清（BCS）对阿霉素（ADR）在艾氏腹水瘤细胞（野生型EAT细胞）及其ADR耐药株细胞内蓄积的影响。在不含BCS的培养基中处理的ADR蓄积量是含10% BCS培养基中的3倍。在无BCS的情况下，进入细胞的ADR不会外流，而在两种细胞株中，当存在10% BCS时，ADR在30分钟内迅速外流至细胞内蓄积量的约20%。这表明BCS由于促进了ADR外流而显著降低了ADR的蓄积。此外，在两种细胞株中，ADR的蓄积量均随BCS浓度的增加而降低。这表明BCS促进ADR外流，且依赖于其浓度。将BCS在100℃加热7分钟，其蛋白质成分变性。它对野生型和ADR耐药株中ADR蓄积的影响很明显。在两种细胞株中，加热后的BCS和未处理的BCS一样，随着其浓度的增加，ADR的蓄积量降低。因此，BCS的蛋白质成分不影响ADR的蓄积。所以，有必要研究BCS的哪些成分会影响ADR外流的促进作用。
