Kleniewski J
Endocrinol Exp. 1975 Sep;9(3):223-7.
The influence of various components of plasma hemostatic system on the assimilation of glucose by isolated rat hemidiaphragm in the presence of insulin was investigated. The addition of streptokinase and human plasminogen to the incubation medium decreased the rate of glucose assimilation. This effect did not occur when trasylol, an inhibitor of plasmin, was added to the assay system. The glucose assimilation by hemidiaphragms incubated in buffer with glucose and insulin was not influenced by thrombin, fibrinopeptides, fibrin degradation products, plasminogen, streptokinase or trasylol added separately to the assay system. The mechanism of the influence of plasmin on insulin action was partly elucidated in further experiments. Thus, less 125I-insulin was bound by isolated fat cells preincubated with plasmin than by control cells.
研究了血浆止血系统的各种成分在胰岛素存在的情况下对分离的大鼠半膈肌葡萄糖同化作用的影响。向孵育培养基中添加链激酶和人纤溶酶原会降低葡萄糖同化率。当向测定系统中添加纤溶酶抑制剂抑肽酶时，这种效应不会出现。在含有葡萄糖和胰岛素的缓冲液中孵育的半膈肌对葡萄糖的同化作用不受分别添加到测定系统中的凝血酶、纤维蛋白肽、纤维蛋白降解产物、纤溶酶原、链激酶或抑肽酶的影响。在进一步的实验中，部分阐明了纤溶酶对胰岛素作用的影响机制。因此，与对照细胞相比，预先用纤溶酶孵育的分离脂肪细胞结合的125I胰岛素较少。
