Smith L J, Sogh J A, Kindt T J, Mandy W J
Eur J Immunol. 1979 Jan;9(1):27-31. doi: 10.1002/eji.1830090107.
Rabbit kappa light (L) chain allotypes are controlled by the allelic genes b4, b5, b6, b9 and a recently described allele b4v. The product of the b4v gene differs from that of b4 by at least two amino acid substitutions in the constant region. A serologic distinction between the b4 and b4v allotyes has now been detected by a radioimmune assay using one of six anti-b4 sera tested. The distinction was enhanced by fractionation of this antiserum on an immunoadsorbent containing cottontail rabbit IgG. IgG with L chains of the b4v type are deficient in their ability to inhibit binding of b4 IgG to the antibody preparation. L chains isolated from b4 and b4v IgG samples could not be distinguished by this assay indicating that interaction with an H chain is required for expression of the L chain determinants that differentiate b4 from b4v.
兔κ轻链（L链）同种异型由等位基因b4、b5、b6、b9以及最近描述的等位基因b4v控制。b4v基因的产物与b4基因的产物在恒定区至少有两个氨基酸替换不同。现在，通过使用所测试的六种抗b4血清之一进行放射免疫测定，已检测到b4和b4v同种异型之间的血清学差异。通过在含有棉尾兔IgG的免疫吸附剂上对该抗血清进行分级分离，这种差异得到了增强。具有b4v型L链的IgG在抑制b4 IgG与抗体制剂结合的能力方面存在缺陷。通过该测定无法区分从b4和b4v IgG样品中分离的L链，这表明与重链相互作用是表达区分b4和b4v的L链决定簇所必需的。
