Knight K L, Schweizer M, Pernis B
Eur J Immunol. 1979 Jan;9(1):36-9. doi: 10.1002/eji.1830090109.
The distribution of two heavy chain subgroups, VHa and VHy, on rabbit peripheral blood lymphocytes was examined by double membrane immunofluorescence. Fluroescent anti-a1 and anti-y33 were found to react with separate B cell populations; no doubly stained cells were observed. Further evidence for the independent expression of genes controlling the VHa and VHy subgroups were obtained by neonatal suppression of a 2 or y33 in a2y33/a3y- heterozygous rabbits. Suppression of VHa did not affect the expression of VHy, nor did the suppression of VHy affect the expression of VHa. The expression of a single VH gene per B cell is in marked contrast to the simultaneous expression of multiple CH genes.
通过双膜免疫荧光法检测了两种重链亚群VHa和VHy在兔外周血淋巴细胞上的分布。发现荧光抗α1和抗γ33与不同的B细胞群体发生反应；未观察到双染细胞。通过在α2γ33/α3γ-杂合兔中对α2或γ33进行新生期抑制，获得了控制VHa和VHy亚群基因独立表达的进一步证据。VHa的抑制不影响VHy的表达，VHy的抑制也不影响VHa的表达。每个B细胞单个VH基因的表达与多个CH基因的同时表达形成鲜明对比。
