Chikayama S, Sugano T, Takahashi Y, Ikeda M, Kimura S, Kobayashi Y, Kondo M
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto-city, Japan.
Int J Oncol. 2000 Jun;16(6):1117-24. doi: 10.3892/ijo.16.6.1117.
Relationship between nuclear accumulation of p53 protein and apoptosis induced by both DNA damaging agents (ultraviolet-irradiation and anticancer agents) and heat shock in normal human skin fibroblasts was investigated. Nuclear accumulation of p53 protein and apoptosis were induced dose-dependently by u.v. -irradiation and anticancer agents. Regarding heat shock treatment, intensive nuclear accumulation of p53 protein was induced by treatment at 43 degrees C for 45 min, but, apoptotic cells were never detected. These results indicated that accumulation of p53 protein and apoptosis induced by DNA damaging agents were considered to have a close relationship and the functions of induced p53 protein are different depending on the stimulating factor.
研究了正常人皮肤成纤维细胞中p53蛋白的核积累与DNA损伤剂（紫外线照射和抗癌剂）及热休克诱导的细胞凋亡之间的关系。紫外线照射和抗癌剂可剂量依赖性地诱导p53蛋白的核积累和细胞凋亡。对于热休克处理，在43℃处理45分钟可诱导p53蛋白强烈的核积累，但未检测到凋亡细胞。这些结果表明，DNA损伤剂诱导的p53蛋白积累与细胞凋亡被认为有密切关系，并且诱导的p53蛋白的功能因刺激因子而异。
