Suzuki S, Shimada T
Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, 113-8602, Japan.
Biochem Biophys Res Commun. 2000 May 19;271(3):672-6. doi: 10.1006/bbrc.2000.2690.
An expression vector encoding a chimeric envelope protein composed of CD4 and ecotropic retroviral envelope glycoprotein was constructed with the aim of accomplishing targeted gene transfer into HIV-1-infected cells. The chimeric protein was efficiently expressed and transported to the surfaces of various cell types and supported HIV-1 entry into human cells. A packaging cell line producing retroviral vectors carrying chimeric envelope proteins was then established. The vector particles produced were shown to be capable of specific gene transfer into human cells expressing HIV envelope glycoprotein. Blocking experiments confirmed that the vector particles entered the cells via an interaction between the chimeric and HIV envelope proteins. This targeting vector may thus be a useful tool with which to develop effective gene therapies against HIV infection.
构建了一种表达载体，其编码由CD4和嗜亲性逆转录病毒包膜糖蛋白组成的嵌合包膜蛋白，目的是实现向HIV-1感染细胞的靶向基因转移。嵌合蛋白在多种细胞类型的表面高效表达并转运，支持HIV-1进入人类细胞。随后建立了一个产生携带嵌合包膜蛋白的逆转录病毒载体的包装细胞系。所产生的载体颗粒能够将特定基因转移到表达HIV包膜糖蛋白的人类细胞中。阻断实验证实，载体颗粒通过嵌合蛋白与HIV包膜蛋白之间的相互作用进入细胞。因此，这种靶向载体可能是开发针对HIV感染的有效基因疗法的有用工具。
