小鼠乳腺上皮细胞中钙池调控性钙离子内流的发育变化

Developmental changes in capacitative Ca(2+) entry in mouse mammary epithelial cells.

作者信息

Ichikawa J, Furuya K, Miyata S, Nakashima T, Kiyohara T

机构信息

Kyoto Institute of Technology, Department of Applied Biology, Matsugasaki, Sakyo-ku, Japan.

出版信息

Cell Biochem Funct. 2000 Jun;18(2):147-50. doi: 10.1002/(SICI)1099-0844(200006)18:2<147::AID-CBF864>3.0.CO;2-W.

DOI:10.1002/(SICI)1099-0844(200006)18:2<147::AID-CBF864>3.0.CO;2-W

PMID:10814975

Abstract

Developmental changes in capacitative Ca(2+) entry and Ca(2+) release from intracellular stores were measured using fura-2 fluorescence method during the pregnancy period (day 3-;18) in mouse mammary epithelial cells. Ca(2+) release was identified with the transient intracellular Ca(2+) (Ca(2+)) increase induced by thapsigargin addition in a Ca(2+)-free solution. Capacitative Ca(2+) entry was measured by the transient Ca(2+) increase induced by re-addition of extracellular Ca(2+) after depletion of Ca(2+) stores by thapsigargin. The capacitative Ca(2+) entry was greatest at the early stage of pregnancy (i.e. day 3 of pregnancy) and decreased as pregnancy progressed, while Ca(2+) release remained unchanged throughout the developmental stages. These findings indicate that in contrast to Ca(2+) release, a close correlation exists between capacitative Ca(2+) entry and pregnancy-induced development in mammary epithelial cells.

摘要

在小鼠乳腺上皮细胞孕期（第3天至第18天），使用fura-2荧光法测量了钙池调控性Ca(2+)内流以及细胞内钙库Ca(2+)释放的发育变化。在无钙溶液中加入毒胡萝卜素诱导细胞内Ca(2+)（Ca(2+)）瞬时增加来鉴定Ca(2+)释放。通过毒胡萝卜素耗尽钙库后再加入细胞外Ca(2+)诱导的Ca(2+)瞬时增加来测量钙池调控性Ca(2+)内流。钙池调控性Ca(2+)内流在孕期早期（即孕期第3天）最大，并随着孕期进展而降低，而Ca(2+)释放在整个发育阶段保持不变。这些发现表明，与Ca(2+)释放不同，钙池调控性Ca(2+)内流与乳腺上皮细胞孕期诱导的发育之间存在密切相关性。