Helmstaedter V, Kreppein W, Domschke W, Mitznegg P, Yanaihara N, Wünsch E, Forssmann W G
Gastroenterology. 1979 May;76(5 Pt 1):897-902.
Motilin-immunoreactive cells in the human and monkey duodenum and upper jejunum were investigated by immunofluorescence (IF) and peroxidase-antiperoxidase (PAP) techniques using antibodies against synthetic 13-norleucine motilin and synthetic porcine motilin. Contrary to previous reports, we have demonstrated that motilin-immunoreactive cells are a distinct cell population that does not correspond to 5-hydroxytryptamine-containing enterochromaffin (EC-) cells. EC-cells, indentified by formaldehyde-induced fluorescence (FIF) or by argentaffinity (AA), do not react with either antisera. EC-cells of the monkey, utilizing one method (PAP), reacted to one antiserum very weakly. This reaction was also suppressed by absorption with 13-norleucine motilin. It is suggested that the EC-cells of the monkey contain either a very small amount of motilin or another peptide that exhibits a part of the amino acid sequence of motilin. The discrepancy between these results and those of other authors can be explained by the different reactivity of the antisera used.
运用抗合成13-去甲亮氨酸胃动素和抗合成猪胃动素抗体，通过免疫荧光（IF）和过氧化物酶-抗过氧化物酶（PAP）技术，对人和猴十二指肠及空肠上段中胃动素免疫反应性细胞进行了研究。与先前报道相反，我们已证实胃动素免疫反应性细胞是一个独特的细胞群体，与含5-羟色胺的肠嗜铬（EC-）细胞并不对应。通过甲醛诱导荧光（FIF）或嗜银性（AA）鉴定的EC细胞，与任何一种抗血清均无反应。利用一种方法（PAP）检测发现，猴的EC细胞对一种抗血清有非常微弱的反应。这种反应也会被13-去甲亮氨酸胃动素吸收所抑制。这表明猴的EC细胞要么含有极少量的胃动素，要么含有另一种呈现胃动素部分氨基酸序列的肽。这些结果与其他作者结果之间的差异可以用所用抗血清的不同反应性来解释。
