Vyazmensky M, Barak Z, Chipman D M, Eichler J
Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Comp Biochem Physiol B Biochem Mol Biol. 2000 Feb;125(2):205-10. doi: 10.1016/s0305-0491(99)00170-4.
Whereas the biochemistry of acetohydroxy acid synthase has been extensively studied in bacteria and eukaryotes, relatively little is known about the enzyme in archaea, the third kingdom of life. The present study biochemically characterizes acetohydroxy acid synthase activity in the halophilic archaea Haloferax volcanii. In addressing ion requirements, enzyme inhibition and antibody labeling, the results reveal that, except for its elevated salt requirements, the haloarchaeal enzyme is remarkably similar to its bacterial counterpart.
虽然乙酰羟酸合酶的生物化学性质在细菌和真核生物中已得到广泛研究，但对于生命的第三个界——古生菌中的这种酶却知之甚少。本研究对嗜盐古生菌沃氏嗜盐栖热菌中的乙酰羟酸合酶活性进行了生物化学表征。在研究离子需求、酶抑制和抗体标记方面，结果显示，除了对盐的需求较高外，嗜盐古生菌的这种酶与其细菌对应物非常相似。
