Mattley Y, Leparc G, Potter R, García-Rubio L
Department of Chemistry, University of South Florida, Tampa 33620, USA.
Photochem Photobiol. 2000 May;71(5):610-9. doi: 10.1562/0031-8655(2000)071<0610:LSAAMF>2.0.CO;2.
Multiwavelength ultraviolet-visible (UV-Vis) transmission spectroscopy is a relatively simple technique that can provide considerable quantitative information on the properties of micron and submicron particle suspensions. Two important particle properties are particle size distribution (PSD) and chemical composition. These properties provide characteristics for the identification and classification of biological systems ranging in size and composition from proteins and nucleic acids to cells. By measuring the complete UV-Vis spectrum, the combined scattering and absorption properties are obtained as a function of wavelength. The quantitative evaluation of the size distribution and chemical composition is accomplished through the application of light-scattering theory. This paper reports on the estimation of the optical properties of human blood platelets and their use in the interpretation of platelet UV-Vis spectra within the context of Mie theory. The model developed herein provides reliable and accurate estimates for the PSD and particle number of platelet suspensions. One potential application of this characterization method is in the analysis of platelet activation by thrombin. Quantification of spectral data with respect to average particle size and particle number provides a real-time description of the dramatic changes that accompany the platelet activation process.
多波长紫外可见（UV-Vis）透射光谱法是一种相对简单的技术，它可以提供有关微米和亚微米颗粒悬浮液性质的大量定量信息。两个重要的颗粒性质是粒度分布（PSD）和化学成分。这些性质为从蛋白质、核酸到细胞等大小和组成各异的生物系统的鉴定和分类提供了特征。通过测量完整的紫外可见光谱，可以获得作为波长函数的组合散射和吸收性质。通过应用光散射理论完成对粒度分布和化学成分的定量评估。本文报道了对人体血小板光学性质的估计及其在米氏理论背景下对血小板紫外可见光谱解释中的应用。本文开发的模型为血小板悬浮液的PSD和颗粒数提供了可靠且准确的估计。这种表征方法的一个潜在应用是分析凝血酶对血小板的激活作用。关于平均粒径和颗粒数的光谱数据量化提供了伴随血小板激活过程的显著变化的实时描述。
