Campillo N, Viñas P, López-García I, Hernández-Córdoba M
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
Analyst. 2000 Feb;125(2):313-6. doi: 10.1039/a907596h.
A procedure for the determination of the total content of arsenic in urine, serum and blood by electrothermal atomic absorption spectrometry (ETAAS) is described. Zeeman correction is used to compensate the high background signals. The samples are diluted (1 + 1 for urine and 1 + 3 for both serum and blood samples) in a medium containing 0.1% w/v Triton X-100 before being introduced directly into the furnace. A solution containing 15% w/v hydrogen peroxide, 0.65% w/v nitric acid and 0.5% w/v nickel is also introduced into the atomizer by means of a separate injection. Calibration is carried out against aqueous standards for blood and serum samples and using the standard additions method for urine samples. The detection limit is 20 pg (2 ng ml-1). The reliability of the procedure is checked by analyzing three certified reference materials and by recovery studies.
本文描述了一种用电热原子吸收光谱法（ETAAS）测定尿液、血清和血液中砷总含量的方法。采用塞曼校正来补偿高背景信号。样品在含有0.1%（w/v） Triton X-100的介质中稀释（尿液为1 + 1，血清和血液样品均为1 + 3），然后直接引入炉中。还通过单独进样将含有15%（w/v）过氧化氢、0.65%（w/v）硝酸和0.5%（w/v）镍的溶液引入雾化器。针对血液和血清样品的水溶液标准品进行校准，尿液样品采用标准加入法。检测限为20 pg（2 ng ml-1）。通过分析三种有证标准物质和回收率研究来检验该方法的可靠性。
