Izmailov S F, Kosenko L V, Troitskaya G N, Kudryavtseva N N, Borodenko L I, Dubrovo P N, Russa R, Pietras H, Lorkiewicz Z
Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow.
Acta Biochim Pol. 1999;46(4):1001-9.
Chemical composition of lipopolysaccharide (LPS) isolated from an effective (97) and ineffective (87) strains of R. l. viciae has been determined. LPS preparations from the two strains contained: glucose, galactose, mannose, fucose, arabinose, heptose, glucosamine, galactosamine, quinovosamine, and 3-N-methyl-3,6-dideoxyhexose, as well as glucuronic, galacturonic and 3-deoxyoctulosonic acid. The following fatty acids were identified: 3-OH 14:0, 3-OH 15:0, 3-OH 16:0, 3-OH 18:0 and 27-OH 28:0. The ratio of 3-OH 14:0 to other major fatty acids in LPS 87 was higher that in LPS 97. SDS/PAGE profiles of LPS indicated that, in lipopolysaccharides, relative content of S form LPS I to that of lower molecular mass (LPS II) was much higher in the effective strain 97 than in 87. All types of polysaccharides exo-, capsular-, lipo, (EPS, CPS, LPS, respectively) examined possessed the ability to bind faba bean lectin. The degree of affinity of the host lectin to LPS 87 was half that to LPS 97. Fatty acids (FA) composition from bacteroids and peribacteroid membrane (PBM) was determined. Palmitic, stearic and hexadecenoic acids were common components found in both strains. There was a high content of unsaturated fatty acids in bacteroids as well as in PBM lipids. The unsaturation index in the PBM formed by strain 87 was lower than in the case of strain 97. Higher ratio of 16:0 to 18:1 fatty acids was characteristic for PMB of the ineffective strain.
已测定从豌豆根瘤菌有效菌株（97）和无效菌株（87）中分离出的脂多糖（LPS）的化学组成。来自这两种菌株的LPS制剂含有：葡萄糖、半乳糖、甘露糖、岩藻糖、阿拉伯糖、庚糖、葡糖胺、半乳糖胺、奎诺糖胺和3 - N - 甲基 - 3,6 - 二脱氧己糖，以及葡糖醛酸、半乳糖醛酸和3 - 脱氧辛糖酸。鉴定出以下脂肪酸：3 - OH 14:0、3 - OH 15:0、3 - OH 16:0、3 - OH 18:0和27 - OH 28:0。LPS 87中3 - OH 14:0与其他主要脂肪酸的比例高于LPS 97。LPS的SDS/PAGE图谱表明，在脂多糖中，有效菌株97中S型LPS I与低分子量（LPS II）的相对含量远高于87。所检测的所有类型的多糖，即胞外多糖、荚膜多糖、脂多糖（分别为EPS、CPS、LPS）都具有结合蚕豆凝集素的能力。宿主凝集素对LPS 87的亲和力程度是对LPS 97的一半。测定了类菌体和类菌体周膜（PBM）的脂肪酸（FA）组成。棕榈酸、硬脂酸和十六碳烯酸是两种菌株中常见的成分。类菌体以及PBM脂质中不饱和脂肪酸含量很高。由菌株87形成的PBM中的不饱和度指数低于菌株97。无效菌株的PBM中16:0与18:1脂肪酸的比例更高。
