Quintilio W, Sato R A, Sant'Anna O A, Esteves M I, Sesso A, de Araujo P S, Bueno da Costa M H
Lab. de Microesferas e Lipossomos, C. de Biotecnologia, I. Butantan, Av. Vital Brasil 1500, 05503-900, Butantan, Brazil.
J Control Release. 2000 Jul 3;67(2-3):409-13. doi: 10.1016/s0168-3659(00)00228-5.
Liposomes, as a pharmaceutical formulation must display a long shelf life. The recombinant heat-shock protein from Mycobacterium leprae (18-kDa hsp) or its N-acylated derivative, when entrapped within or externally associated with large unilamellar vesicles, acts as a T-epitope source. Freeze-fracture electron microscopy shows unequivocally that trehalose avoids aggregation and fusion of these vesicles. Formulations containing trehalose retained up to 98% of the entrapped protein. The highest antibody level is obtained with formulations containing trehalose. The adjuvant effect depends on the liposomal membrane integrity.
脂质体作为一种药物制剂必须具有较长的保质期。当来自麻风分枝杆菌的重组热休克蛋白（18-kDa hsp）或其N-酰化衍生物包裹在大单层囊泡内或与其外部结合时，可作为T表位来源。冷冻断裂电子显微镜明确显示海藻糖可避免这些囊泡的聚集和融合。含有海藻糖的制剂可保留高达98%的包裹蛋白。含海藻糖的制剂可获得最高抗体水平。佐剂效应取决于脂质体膜的完整性。
