将编码丝裂原活化蛋白激酶激酶MEK1（MAP2K1）的人类基因定位于15q21，以及将MEK2（MAP2K2）定位于7q32的染色体图谱分析。

Chromosome mapping of the human genes encoding the MAP kinase kinase MEK1 (MAP2K1) to 15q21 and MEK2 (MAP2K2) to 7q32.

作者信息

Meloche S, Gopalbhai K, Beatty B G, Scherer S W, Pellerin J

机构信息

Research Centre, Centre hospitalier de l'Université de Montréal and Department of Pharmacology, University of Montreal, Quebec, Canada.

出版信息

Cytogenet Cell Genet. 2000;88(3-4):249-52. doi: 10.1159/000015530.

DOI:10.1159/000015530

PMID:10828601

Abstract

Activation of the ERK mitogen-activated protein (MAP) kinase pathway has been implicated in the regulation of cell growth, differentiation and senescence. In this pathway, the MAP kinases ERK1/ERK2 are phosphorylated and activated by the dual-specificity kinases MEK1 and MEK2, which in turn are activated by serine phosphorylation by a number of MAP kinase kinase kinases. We report here the chromosomal localization of the human genes encoding the MAP kinase kinase isoforms MEK1 and MEK2. Using a combination of fluorescence in situ hybridization, somatic cell hybrid analysis, DNA sequencing and yeast artificial chromosome (YAC) clone analysis, we have mapped the MEK1 gene (MAP2K1) to chromosome 15q21. We also present evidence for the presence of a MEK1 pseudogene on chromosome 8p21. The MEK2 gene (MAP2K2) was mapped to chromosome 7q32 by fluorescence in situ hybridization and YAC clone analysis.

摘要

细胞外信号调节激酶（ERK）丝裂原活化蛋白（MAP）激酶信号通路的激活与细胞生长、分化及衰老的调控有关。在该信号通路中，MAP激酶ERK1/ERK2被双特异性激酶MEK1和MEK2磷酸化并激活，而MEK1和MEK2又被多种MAP激酶激酶激酶通过丝氨酸磷酸化激活。我们在此报告编码MAP激酶激酶同工型MEK1和MEK2的人类基因的染色体定位。通过荧光原位杂交、体细胞杂种分析、DNA测序及酵母人工染色体（YAC）克隆分析相结合的方法，我们将MEK1基因（MAP2K1）定位于15号染色体q21区。我们还提供了8号染色体p21区存在MEK1假基因的证据。通过荧光原位杂交和YAC克隆分析，将MEK2基因（MAP2K2）定位于7号染色体q32区。