Kuttler B, Wanka H, Hahn H J
Institute of Pathophysiology Karlsburg, Medical School of the Ernst-Moritz-Arndt-University of Greifswald, Karlsburg, Germany.
Transplantation. 2000 May 15;69(9):1987-90. doi: 10.1097/00007890-200005150-00047.
A co-culture of splenic lymphocytes with allogeneic pancreatic islets [i.e., mixed lymphocyte islet co-culture (MLIC)] for 96 hr leads to reduction of beta-cells and to an allospecific induction of major histocompatibility complex (MHC) class II antigens on beta-cells. The intent of our investigation was to determine whether peripheral blood lymphocytes (PBL) obtained from allogeneic islet-grafted BB/OK rats (=sensitization in vivo) cause similar alterations to donor-specific islet cells. PBL prepared before transplantation, before (at day 7) and after islet rejection were co-cultured for 24 hr with donor-specific islets. PBL obtained at any time before and after transplantation caused reduction of beta-cells and enhancement of intercellular adhesion molecule-1(+)/beta-cells. Induction of MHC class II+ beta-cells was most pronounced with PBL obtained before rejection. Down-regulation of major histocompatibility complex class I+ beta-cells was caused by PBL that had been obtained from grafted animals only; it was most pronounced before islet rejection and has never been observed with lymphocytes from nongrafted normoglycemic rats. The 24-hr MLIC is capable of recognizing functionally active, donor-specific lymphocytes and is able to distinguish between the effects of sensitized and nonsensitized lymphocytes.
脾淋巴细胞与同种异体胰岛进行共培养（即混合淋巴细胞胰岛共培养，MLIC）96小时会导致β细胞数量减少，并使β细胞上主要组织相容性复合体（MHC）II类抗原出现同种特异性诱导。我们研究的目的是确定从同种异体胰岛移植的BB/OK大鼠获取的外周血淋巴细胞（PBL）（即体内致敏）是否会对供体特异性胰岛细胞产生类似的改变。在移植前、胰岛排斥前（第7天）和排斥后制备的PBL与供体特异性胰岛共培养24小时。在移植前后任何时间获取的PBL都会导致β细胞数量减少，并使细胞间黏附分子-1阳性/β细胞增加。MHC II类阳性β细胞的诱导在排斥前获取的PBL中最为明显。主要组织相容性复合体I类阳性β细胞的下调仅由从移植动物获取的PBL引起；在胰岛排斥前最为明显，从未在未移植的血糖正常大鼠的淋巴细胞中观察到这种情况。24小时的MLIC能够识别功能活跃的供体特异性淋巴细胞，并能够区分致敏淋巴细胞和未致敏淋巴细胞的作用。
