Hoffmann F, Harnisch J P
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1976 Jan 16;198(1):63-72. doi: 10.1007/BF00411446.
Thirty rabbits were divided into 10 groups of 3 animals. In the case of each animal 1 drop of Herpes virus hominis suspension (HVH) was applied to the intact cornea of the right eye. Within the period between the 2nd and 20th day after infection occurred, three animals were killed each time and the corneae were examined by scanning- and transmission-electron microscope. Circular foci and dendritic lesions were observed starting with the 4th day. After the 12th day, dendritic lesions could no longer be observed. Circular foci and dendritic lesions possess a width of about 200 mu which remains unchanged during the entire observation period. The areas of ulceration are obscured by lateral epithelium migrations. The observation that epithelial lesions do not change their demensions over an extended period of time, speaks for an approximate time balance between cellular damage caused by the viruses and the migratory speed of the epithelium. This mechanism is disrupted in the neighboring circular foci. The cytotoxic effect is predominant. Circular foci are confluent. The dendritic lesion so characteristic for herpes simplex infection of the cornea is explained by the confluence of several circular foci which are accidentally positioned next to each other.
30只兔子被分成10组，每组3只。对每只动物的右眼完整角膜滴加1滴人疱疹病毒悬液（HVH）。在感染后的第2天至第20天期间，每次处死3只动物，并用扫描电子显微镜和透射电子显微镜检查角膜。从第4天开始观察到圆形病灶和树枝状病变。第12天后，不再观察到树枝状病变。圆形病灶和树枝状病变的宽度约为200微米，在整个观察期内保持不变。溃疡区域被上皮细胞侧向迁移所遮盖。上皮病变在较长时间内尺寸不变，这一观察结果表明病毒引起的细胞损伤与上皮细胞迁移速度之间存在近似的时间平衡。在相邻的圆形病灶中，这种机制被破坏。细胞毒性作用占主导。圆形病灶相互融合。角膜单纯疱疹感染特有的树枝状病变是由几个偶然相邻的圆形病灶融合形成的。
