Nakabayashi N, Hiranuma K
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Dent Mater. 2000 Jul;16(4):274-9. doi: 10.1016/s0109-5641(00)00017-8.
To collect data that explains the advantage, if any, of wet bonding versus dry bonding to dentin, and to more clearly understand the mechanism of wet bonding.
Bovine dentin samples were prepared with #600-grit paper and were divided into four groups of six each. The first six specimens were etched with 10% citric acid and 3% ferric chloride for 10 s then rinsed and blot-dried (Gr. 1: 10-3:W). The second six were etched with 10% citric acid and 3% ferric chloride then rinsed and air-dried (Gr. 2: 10-3:D). The third six were etched with 10% citric acid for 10 s, rinsed and blot-dried (Gr. 3: 10-0:W). The fourth group of six samples was etched, rinsed and air-dried (Gr. 4: 10-0:D). All groups were primed with 5% 4-methacryloyloxyethyl trimellitate anhydride (4-META) in acetone for 60 s and an acrylic rod was bonded to the samples using a 4-META/methyl methacrylate (MMA)-tri-n-butyl borane (TBB) resin. The samples were fashioned into dumbbell-shaped specimens and stressed in tension until bond failure, to determine tensile bond strengths. Fractured surfaces were examined by scanning electron microscopy.
The mean tensile bond strengths of specimen groups were found to be 18.9 (8.1) MPa (Gr. 1: 10-3:W), 18.1 (1.7) MPa (Gr. 2: 10-3:D), 11.9 (4.4) MPa (Gr. 3: 10-0:W) and 5.4 (2.8) MPa (Gr. 4: 10-0:D). There was no statistically significant difference between Grs. 1 and 2 (p > 0.01), or between Grs. 1 and 3 (p > 0.05). The TBS of Gr. 4 (10-0:D) was significantly lower than the others (p < 0.01). The 5% 4-META in acetone primer was apparently quite effective in improving monomer impregnation into demineralized dentin resulting in increased resin content within the hybridized dentin.
Effective dentin bonding depended upon the etchants employed. 10-0 etching and air-drying caused the demineralized dentin to collapse in which case wet bonding became necessary to obtain good TBS data. The specimens demineralized with 10-3 did not collapse, even when air-dried; consequently both wet and dry bonding proved effective for obtaining high tensile bond strength data.
收集能解释湿粘结与干粘结相比（若有）对牙本质的优势的数据，并更清楚地了解湿粘结的机制。
用600目砂纸制备牛牙本质样本，并将其分为四组，每组六个。前六个样本用10%柠檬酸和3%氯化铁蚀刻10秒，然后冲洗并吸干（第1组：10 - 3:W）。第二组六个样本用10%柠檬酸和3%氯化铁蚀刻，然后冲洗并吹干（第2组：10 - 3:D）。第三组六个样本用10%柠檬酸蚀刻10秒，冲洗并吸干（第3组：10 - 0:W）。第四组六个样本蚀刻、冲洗并吹干（第4组：10 - 0:D）。所有组均用5%丙酮中的4 - 甲基丙烯酰氧乙基偏苯三酸酐（4 - META）灌注60秒，并用4 - META/甲基丙烯酸甲酯（MMA）-三正丁基硼烷（TBB）树脂将丙烯酸棒粘结到样本上。将样本制成哑铃形试件，并进行拉伸应力直至粘结失效，以测定拉伸粘结强度。通过扫描电子显微镜检查断裂表面。
发现样本组的平均拉伸粘结强度分别为18.9（8.1）MPa（第1组：10 - 3:W）、18.1（1.7）MPa（第2组：10 - 3:D）、11.9（4.4）MPa（第3组：10 - 0:W）和5.4（2.8）MPa（第4组：10 - 0:D）。第1组和第2组之间（p > 0.01）或第1组和第3组之间（p > 0.05）无统计学显著差异。第4组（10 - 0:D）的TBS显著低于其他组（p < 0.01）。丙酮中的5% 4 - META灌注剂在改善单体渗入脱矿牙本质方面显然相当有效，从而导致杂交牙本质中树脂含量增加。
有效的牙本质粘结取决于所使用的蚀刻剂。10 - 0蚀刻和吹干会导致脱矿牙本质塌陷，在这种情况下，湿粘结对于获得良好的TBS数据变得必要。用10 - 3脱矿的样本即使吹干也不会塌陷；因此，湿粘结和干粘结对于获得高拉伸粘结强度数据都被证明是有效的。
