Department of Restorative Dentistry, Hokkaido University Graduate School of Dentistry, Sapporo, Japan.
J Adhes Dent. 2011 Oct;13(5):411-6. doi: 10.3290/j.jad.a19818.
To investigate the effect of Colloidal Platinum Nanoparticles (CPN) on the bond strength between dentin and 4-META/MMA-TBB resin using different concentrations of CPN.
Twenty-five extracted human third molars were stored in 0.5% chloramine T. The occlusal dentin slices were prepared by grinding occlusal surfaces of each tooth and polishing with 600-grit silicon carbide paper under running water. One control and four experimental groups (2 specimens per group) were used as follows: a) dentin surfaces treated with 10-3 solution, followed by rinsing with water and subsequently an acrylic rod bonded with hand-mixed 4META/MMA-TBB resin (Super-Bond C&B, Sun Medical) (control); b) dentin surfaces treated with 10-3 etching solution, followed by rinsing with water and application of CPN (100% or 10%) as a primer solution for 60 s and rinsed with water for 20 s, then an acrylic rod bonded with Super-Bond C&B(Etch-CPN [100% or 10%]); c) dentin surfaces treated with CPN (100% or 10%) for 60 s, rinsed with water for 20 s, followed by application of 10-3 solution, then an acrylic rod bonded with Super-Bond C&B (CPN-Etch [100% or 10%]). After storage in 37°C water, specimens were sectioned into beams (cross-sectional area: 1 mm2) for microtensile bond strength testing at a crosshead speed of 1mm/min. The data were analyzed using the Games-Howell method (p < 0.05; n = 15).
Etch-CPN (100), CPN-Etch(100) and CPN-Etch (10) showed significantly higher bond strengths compared to the control. When using 10% CPN, the highest bond strength was demonstrated. The bond strength of 4META/MMA-TBB resin was approximately doubled by CPN application.
The results of this study showed that higher bond strengths are obtained when treating dentin with a lower concentration of CPN. Further evaluation to optimize conditions such as the application time and rinsing time are required.
研究不同浓度胶体铂纳米粒子（CPN）对牙本质与 4-META/MMA-TBB 树脂粘结强度的影响。
将 25 颗人第三磨牙浸泡在 0.5%的氯胺 T 中。通过磨除每颗牙齿的咬合面并在流水下用 600 号碳化硅砂纸进行抛光来制备牙本质切盘。使用一个对照组和四个实验组（每组 2 个标本），具体如下：a）用 10-3 溶液处理牙本质表面，然后用流水冲洗，随后用手动混合的 4-META/MMA-TBB 树脂（Super-Bond C&B，Sun Medical）粘结丙烯酸棒（对照组）；b）用 10-3 蚀刻溶液处理牙本质表面，然后用流水冲洗，然后用 CPN（100%或 10%）作为底涂溶液处理 60 秒，并用流水冲洗 20 秒，然后用 Super-Bond C&B 粘结丙烯酸棒（Etch-CPN [100%或 10%]）；c）用 CPN（100%或 10%）处理牙本质表面 60 秒，用流水冲洗 20 秒，然后用 10-3 溶液处理，再用 Super-Bond C&B 粘结丙烯酸棒（CPN-Etch [100%或 10%]）。在 37°C 水中储存后，将标本切割成梁（横截面面积：1mm2），以 1mm/min 的十字头速度进行微拉伸粘结强度测试。使用 Games-Howell 方法分析数据（p<0.05；n=15）。
与对照组相比，Etch-CPN（100）、CPN-Etch（100）和 CPN-Etch（10）显示出更高的粘结强度。当使用 10%CPN 时，粘结强度最高。CPN 的应用使 4-META/MMA-TBB 树脂的粘结强度增加了约一倍。
本研究结果表明，用较低浓度的 CPN 处理牙本质可获得更高的粘结强度。需要进一步评估以优化应用时间和冲洗时间等条件。
