Boguslawski G, Lyons D, Harvey K A, Kovala A T, English D
Experimental Cell Research Program, Clarian Health Partners, Inc., Indianapolis, Indiana 46202, USA.
Biochem Biophys Res Commun. 2000 Jun 7;272(2):603-9. doi: 10.1006/bbrc.2000.2822.
Sphingosylphosphorylcholine (SPC) is one of the biologically active phospholipids that may act as extracellular messengers. Particularly important is the role of these lipids in the angiogenic response, a complex process involving endothelial cell migration, proliferation, and morphologic differentiation. Here we demonstrate that SPC and its hydrolytic product, sphingosine, induce chemotactic migration of human and bovine endothelial cells. The response is approximately equal to that elicited by vascular endothelial cell growth factor. The effect of SPC and sphingosine was associated with a rapid down-regulation of Edg1, a sphingosine 1-phosphate (SPP)-specific receptor involved in endothelial cell chemotaxis. Both SPC and sphingosine induced differentiation of endothelial cells into capillary-like structures in vitro. Thus, SPC and sphingosine join SPP among the biologically active lipids with angiogenic potential. Since neuronal abnormalities accompany pathological accumulation of SPC in brain tissue, it is possible that SPC is a modulator of angiogenesis in neural tissue upon its release from brain cells following trauma or neoplastic growth.
鞘氨醇磷酸胆碱（SPC）是一种具有生物活性的磷脂，可作为细胞外信使发挥作用。这些脂质在血管生成反应中的作用尤为重要，血管生成是一个复杂的过程，涉及内皮细胞迁移、增殖和形态分化。在此，我们证明SPC及其水解产物鞘氨醇可诱导人和牛内皮细胞的趋化性迁移。该反应与血管内皮细胞生长因子引发的反应大致相同。SPC和鞘氨醇的作用与Edg1的快速下调有关，Edg1是一种参与内皮细胞趋化性的鞘氨醇-1-磷酸（SPP）特异性受体。SPC和鞘氨醇均可在体外诱导内皮细胞分化为毛细血管样结构。因此，SPC和鞘氨醇与具有血管生成潜力的生物活性脂质中的SPP一样。由于神经元异常伴随着SPC在脑组织中的病理性积累，因此在创伤或肿瘤生长后SPC从脑细胞释放时，它有可能是神经组织中血管生成的调节剂。
